ArticleMethods in molecular biology (Clifton, N.J.)2012
Revealing the transcriptome landscape of mouse spermatogonial cells by tiling microarray.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis.Reproduction (Cambridge, England) · 2017Article
- GermlncRNA: a unique catalogue of long non-coding RNAs and associated regulations in male germ cell development.Database : the journal of biological databases and curation · 2015Article
- Transcriptome profiling of the murine testis during the first wave of spermatogenesis.PloS one · 2013Article
- Sperm proteomics: road to male fertility and contraception.International journal of endocrinology · 2013Review
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3 authors.
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Abstract
In the past decade, the advent of microarray technologies has allowed functional genomic studies of male germ cell development, resulting in the identification of genes governing various processes. A major limitation with conventional gene expression microarray is that results obtained are biased due to gene probe design. The gene probes for expression microarrays are usually represented by a small number of probes located at the 3' end of a transcript. Tiling microarrays eliminate such issue by interrogating the genome in an unbiased fashion through probes tiled across the entire genome. These arrays provide higher genomic resolution and allow the identification of novel transcripts. To reveal the complexity of the genomic landscape of developing male germ cells, we applied tiling microarray to evaluate the transcriptome in spermatogonial cells. Over 50% of all known mouse genes are expressed during testicular development. More than 47% of the transcripts are uncharacterized. The results suggested that the transcription machinery in spermaotogonial cells is more complex than previously envisioned.
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