ArticleProceedings of the National Academy of Sciences of the United States of America2011
Computational design of a symmetric homodimer using β-strand assembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Fasciclin 2 Cooperates with Discs Large to Maintain Epithelial Architecture.bioRxiv : the preprint server for biology · 2026Article
- Design of amyloidogenic peptide traps.Nature chemical biology · 2024Article
- Targeting Ras with protein engineering.Oncotarget · 2023Review
- Reconfigurable asymmetric protein assemblies through implicit negative design.Science (New York, N.Y.) · 2022Article
- Antibody interfaces revealed through structural mining.Computational and structural biotechnology journal · 2022Article
- Protein Assembly by Design.Chemical reviews · 2021Review
- Transferrin receptor targeting by de novo sheet extension.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Computational design and experimental optimization of protein binders with prospects for biomedical applications.Protein engineering, design & selection : PEDS · 2021Review
- Self-assembly and regulation of protein cages from pre-organised coiled-coil modules.Nature communications · 2021Article
- Second career of a biosynthetic enzyme: Lumazine synthase as a virus-like nanoparticle in vaccine development.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Engineering protein assemblies with allosteric control via monomer fold-switching.Nature communications · 2019Article
- A threonine zipper that mediates protein-protein interactions: Structure and prediction.Protein science : a publication of the Protein Society · 2018Article
- Creation of artificial protein-protein interactions using α-helices as interfaces.Biophysical reviews · 2018Review
- Cyclic oligomer design with de novo αβ-proteins.Protein science : a publication of the Protein Society · 2017Article
- Computational design of self-assembling cyclic protein homo-oligomers.Nature chemistry · 2017Article
- Accurate design of megadalton-scale two-component icosahedral protein complexes.Science (New York, N.Y.) · 2016Article
- The Folding of de Novo Designed Protein DS119 via Molecular Dynamics Simulations.International journal of molecular sciences · 2016Article
- Engineering a genetically encoded competitive inhibitor of the KEAP1-NRF2 interaction via structure-based design and phage display.Protein engineering, design & selection : PEDS · 2016Article
- Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.Protein science : a publication of the Protein Society · 2015Article
- Computational design and experimental verification of a symmetric protein homodimer.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Computational design of novel protein-protein interfaces is a test of our understanding of protein interactions and has the potential to allow modification of cellular physiology. Methods for designing high-affinity interactions that adopt a predetermined binding mode have proved elusive, suggesting the need for new strategies that simplify the design process. A solvent-exposed backbone on a β-strand is thought of as "sticky" and β-strand pairing stabilizes many naturally occurring protein complexes. Here, we computationally redesign a monomeric protein to form a symmetric homodimer by pairing exposed β-strands to form an intermolecular β-sheet. A crystal structure of the designed complex closely matches the computational model (rmsd = 1.0 Å). This work demonstrates that β-strand pairing can be used to computationally design new interactions with high accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.