ArticleProceedings of the National Academy of Sciences of the United States of America2011
Local adaptation of an introduced transgenic insect fungal pathogen due to new beneficial mutations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Transposable elements hitchhike on Starships across fungal genomes.Nature communications · 2026Article
- Harnessing Nutritional Niches to Explore Fungus-Animal Symbioses.Ecology letters · 2025Review
- Unveiling a novel entry gate: Insect foregut as an alternative infection route for fungal entomopathogens.Innovation (Cambridge (Mass.)) · 2024Article
- Frequent horizontal chromosome transfer between asexual fungal insect pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Diploidy within a Haploid Genus of Entomopathogenic Fungi.Genome biology and evolution · 2021Article
- Empirical Support for the Pattern of Competitive Exclusion between Insect Parasitic Fungi.Journal of fungi (Basel, Switzerland) · 2021Article
- Review
- Population genomics and evolution of a fungal pathogen after releasing exotic strains to control insect pests for 20 years.The ISME journal · 2020Article
- Duplication of a Pks gene cluster and subsequent functional diversification facilitate environmental adaptation in Metarhizium species.PLoS genetics · 2018Article
- Article
- Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes.Scientific reports · 2017Article
- The Insect Pathogens.Microbiology spectrum · 2017Review
- Article
- Identification of Drosophila Mutants Affecting Defense to an Entomopathogenic Fungus.Scientific reports · 2015Article
- Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci.BMC biotechnology · 2015Article
- Expression of scorpion toxin LqhIT2 increases the virulence of Metarhizium acridum towards Locusta migratoria manilensis.Journal of industrial microbiology & biotechnology · 2014Article
- The genome sequence of the biocontrol fungus Metarhizium anisopliae and comparative genomics of Metarhizium species.BMC genomics · 2014Article
- Article
- A fungal insecticide engineered for fast per os killing of caterpillars has high field efficacy and safety in full-season control of cabbage insect pests.Applied and environmental microbiology · 2013Article
- Genetic approaches to interfere with malaria transmission by vector mosquitoes.Trends in biotechnology · 2013Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically modified Metarhizium spp represent a major new arsenal for combating insect pests and insect-borne diseases. However, for these tools to be used safely and effectively, we need a much better understanding of their evolutionary potential and invasion ecology. In order to model natural as well as anthropogenic dispersal scenarios, we investigated evolutionary processes in a green fluorescent protein tagged strain of Metarhizium robertsii following transfer from a semitropical to a temperate soil community. Adaptive changes occurred over four years despite recurrent genetic bottlenecks and lack of recombination with locally well adapted strains. By coupling microarray-based functional analysis with DNA hybridizations we determined that expression of cell wall and stress response genes evolved at an accelerated rate in multiple replicates, whereas virulence determinants, transposons, and chromosome structure were unaltered. The mutable genes were enriched for TATA boxes possibly because they are larger mutational targets. In further field trials, we showed that the new mutations increased the fitness of M. robertsii in the new range by enhancing saprophytic associations, and these benefits were maintained in subsequent years. Consistent with selection being habitat rather than host specific, populations of an avirulent mutant cycled with seasons similarly to the wild type, whereas a mutant unable to adhere to plant roots showed a linear decrease in population. Our results provide a mechanistic basis for understanding postrelease adaptations, show that agents can be selected that lack gene flow and virulence evolution, and describe a means of genetically containing transgenic strains by disrupting the Mad2 gene.
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