ArticlePLoS neglected tropical diseases2011
Inhibition of dengue virus entry and multiplication into monocytes using RNA interference.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Cellular Biology: Virus-Host Interactions and RNA Structure and Function.Current topics in microbiology and immunology · 2026Review
- Interplay between innate immune cells and inflammatory mediators in dengue infection: an evolving therapeutic paradigm.Expert reviews in molecular medicine · 2025Review
- Novel and repurposed antiviral molecules for arbovirus infections with epidemic Potential: A systematic review.New microbes and new infections · 2025Review
- A comprehensive overview on the crosstalk between microRNAs and viral pathogenesis and infection.Medicinal research reviews · 2025Review
- Evaluation of Four Humanized NOD-Derived Mouse Models for Dengue Virus-2 Infection.Pathogens (Basel, Switzerland) · 2024Article
- Elevated TNF-α Induces Thrombophagocytosis by Mononuclear Cells in ex vivo Whole-Blood Co-Culture with Dengue Virus.Journal of inflammation research · 2022Article
- A Preliminary Investigation on the Antiviral Activities of the Philippine Marshmint (Mentha arvensis) Leaf Extracts against Dengue Virus Serotype 2 In Vitro.The Kobe journal of medical sciences · 2021Article
- Amaryllidaceae Alkaloid Cherylline Inhibits the Replication of Dengue and Zika Viruses.Antimicrobial agents and chemotherapy · 2021Article
- Computational prediction of potential siRNA and human miRNA sequences to silence orf1ab associated genes for future therapeutics against SARS-CoV-2.Informatics in medicine unlocked · 2021Article
- Review
- Early Events in Japanese Encephalitis Virus Infection: Viral Entry.Pathogens (Basel, Switzerland) · 2018Review
- Blockade of dengue virus entry into myeloid cells by endocytic inhibitors in the presence or absence of antibodies.PLoS neglected tropical diseases · 2018Article
- Discriminating Clinical and Biological Features in Malaria and Dengue Patients.Journal of arthropod-borne diseases · 2018Article
- Flavivirus Receptors: Diversity, Identity, and Cell Entry.Frontiers in immunology · 2018Review
- Potential for evolution of complex defense strategies in a multi-scale model of virus-host coevolution.BMC evolutionary biology · 2016Article
- Tackling dengue fever: Current status and challenges.Virology journal · 2015Review
- A Molecular Approach Designed to Limit the Replication of Mature DENV2 in Host Cells.Viral immunology · 2015Article
- Current neurological observations and complications of dengue virus infection.Current neurology and neuroscience reports · 2015Review
- SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Dengue-3 Virus Entry into Vero Cells: Role of Clathrin-Mediated Endocytosis in the Outcome of Infection.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDengue infection ranks as one of the most significant viral diseases of the globe. Currently, there is no specific vaccine or antiviral therapy for prevention or treatment. Monocytes/macrophages are the principal target cells for dengue virus and are responsible for disseminating the virus after its transmission. Dengue virus enters target cells via receptor-mediated endocytosis after the viral envelope protein E attaches to the cell surface receptor. This study aimed to investigate the effect of silencing the CD-14 associated molecule and clathrin-mediated endocytosis using siRNA on dengue virus entry into monocytes. METHODOLOGY/PRINCIPAL
findingsGene expression analysis showed a significant down-regulation of the target genes (82.7%, 84.9 and 76.3% for CD-14 associated molecule, CLTC and DNM2 respectively) in transfected monocytes. The effect of silencing of target genes on dengue virus entry into monocytes was investigated by infecting silenced and non-silenced monocytes with DENV-2. Results showed a significant reduction of infected cells (85.2%), intracellular viral RNA load (73.0%), and extracellular viral RNA load (63.0%) in silenced monocytes as compared to non-silenced monocytes. CONCLUSIONS/SIGNIFICANCE: Silencing the cell surface receptor and clathrin mediated endocytosis using RNA interference resulted in inhibition of the dengue virus entry and subsequently multiplication of the virus in the monocytes. This might serve as a novel promising therapeutic target to attenuate dengue infection and thus reduce transmission as well as progression to severe dengue hemorrhagic fever.
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