ArticlePloS one2011
Inducible gene manipulations in brain serotonergic neurons of transgenic rats.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- 2015 Guidelines for Establishing Genetically Modified Rat Models for Cardiovascular Research.Journal of cardiovascular translational research · 2015Guideline
- Behavioral impact of chemogenetic manipulations of 5-HT DRN neurons in transgenic Tph2-iCre rats.Psychopharmacology · 2026Article
- Functional behavioral profiling of multiple Cre-driver rat lines.Experimental animals · 2026Article
- Optogenetic Stimulation of Novel Tph2-Cre Rats Advances Insight into Serotonin's Role in Locomotion, Reinforcement, and Compulsivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Exploring the effects of adolescent social isolation stress on the serotonin system and ethanol-motivated behaviors.Psychopharmacology · 2025Article
- Role of serotonin neurons in the dorsal raphe nucleus in heroin self-administration and punishment.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2025Article
- Sex differences in ethanol consumption and drinking despite negative consequences following adolescent social isolation stress in male and female rats.Physiology & behavior · 2023Article
- Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases.Transgenic research · 2021Article
- Manipulation of dorsal raphe serotonergic neurons modulates active coping to inescapable stress and anxiety-related behaviors in mice and rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2019Article
- Forebrain-specific, conditional silencing of Staufen2 alters synaptic plasticity, learning, and memory in rats.Genome biology · 2017Article
- Transgenic mice lacking CREB and CREM in noradrenergic and serotonergic neurons respond differently to common antidepressants on tail suspension test.Scientific reports · 2017Article
- Divergent in vivo activity of non-serotonergic and serotonergic VGluT3-neurones in the median raphe region.The Journal of physiology · 2016Article
- Chronic central serotonin depletion attenuates ventilation and body temperature in young but not adult Tph2 knockout rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- Tissue Specific Expression of Cre in Rat Tyrosine Hydroxylase and Dopamine Active Transporter-Positive Neurons.PloS one · 2016Article
- Neurodevelopmental Malformations of the Cerebellar Vermis in Genetically Engineered Rats.Cerebellum (London, England) · 2015Article
- Losing Control: Excessive Alcohol Seeking after Selective Inactivation of Cue-Responsive Neurons in the Infralimbic Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Defining functional gene-circuit interfaces in the mouse nervous system.Genes, brain, and behavior · 2014Review
- Whole-rat conditional gene knockout via genome editing.Nature methods · 2013Article
- New tricks for old dogmas: optogenetic and designer receptor insights for Parkinson's disease.Brain research · 2013Review
- Synthetic microRNA-mediated downregulation of Nogo-A in transgenic rats reveals its role as regulator of synaptic plasticity and cognitive function.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The serotonergic (5-HT) system has been implicated in various physiological processes and neuropsychiatric disorders, but in many aspects its role in normal and pathologic brain function is still unclear. One reason for this might be the lack of appropriate animal models which can address the complexity of physiological and pathophysiological 5-HT functioning. In this respect, rats offer many advantages over mice as they have been the animal of choice for sophisticated neurophysiological and behavioral studies. However, only recently technologies for the targeted and tissue specific modification of rat genes - a prerequisite for a detailed study of the 5-HT system - have been successfully developed. Here, we describe a rat transgenic system for inducible gene manipulations in 5-HT neurons. We generated a Cre driver line consisting of a tamoxifen-inducible CreERT2 recombinase under the control of mouse Tph2 regulatory sequences. Tissue-specific serotonergic Cre recombinase expression was detected in four transgenic TPH2-CreERT2 rat founder lines. For functional analysis of Cre-mediated recombination, we used a rat Cre reporter line (CAG-loxP.EGFP), in which EGFP is expressed after Cre-mediated removal of a loxP-flanked lacZ STOP cassette. We show an in-depth characterisation of this rat Cre reporter line and demonstrate its applicability for monitoring Cre-mediated recombination in all major neuronal subpopulations of the rat brain. Upon tamoxifen induction, double transgenic TPH2-CreERT2/CAG-loxP.EGFP rats show selective and efficient EGFP expression in 5-HT neurons. Without tamoxifen administration, EGFP is only expressed in few 5-HT neurons which confirms minimal background recombination. This 5-HT neuron specific CreERT2 line allows Cre-mediated, inducible gene deletion or gene overexpression in transgenic rats which provides new opportunities to decipher the complex functions of the mammalian serotonergic system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.