ArticlePloS one2011
Identification of a TPX2-like microtubule-associated protein in Drosophila.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 63 citations in OpenAlex.
- Capillary bundling of microtubules by condensates.bioRxiv : the preprint server for biology · 2026Article
- Identification of locally activated spindle-associated proteins in oocytes uncovers a phosphatase-driven mechanism.Journal of cell science · 2025Article
- The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis.The Plant cell · 2025Article
- Identification of hypoxia-related diagnostic biomarkers and immune signatures in diminished ovarian reserve.Frontiers in genetics · 2025Article
- HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.Nature communications · 2024Article
- HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.bioRxiv : the preprint server for biology · 2023Article
- Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.The Journal of cell biology · 2023Article
- Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.Journal of cell science · 2023Article
- Augmin is a Ran-regulated spindle assembly factor.The Journal of biological chemistry · 2023Article
- How Microtubules Build the Spindle Branch by Branch.Annual review of cell and developmental biology · 2022Review
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- Article
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- A guide to plant TPX2-like and WAVE-DAMPENED2-like proteins.Journal of experimental botany · 2021Article
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- Ancient origins of allosteric activation in a Ser-Thr kinase.Science (New York, N.Y.) · 2020Article
- Direct observation of branching MT nucleation in living animal cells.The Journal of cell biology · 2019Article
- Review
- TPX2-LIKE PROTEIN3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis.Plant physiology · 2019Article
- Spindle assembly in egg extracts of the Marsabit clawed frog, Xenopus borealis.Cytoskeleton (Hoboken, N.J.) · 2018Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome segregation during mitosis and meiosis relies on the spindle and the functions of numerous microtubule-associated proteins (MAPs). One of the best-studied spindle MAPs is the highly conserved TPX2, which has been reported to have characteristic intracellular dynamics and molecular activities, such as nuclear localisation in interphase, poleward movement in the metaphase spindle, microtubule nucleation, microtubule stabilisation, microtubule bundling, Aurora A kinase activation, kinesin-5 binding, and kinesin-12 recruitment. This protein has been shown to be essential for spindle formation in every cell type analysed so far. However, as yet, TPX2 homologues have not been found in the Drosophila genome. In this study, I found that the Drosophila protein Ssp1/Mei-38 has significant homology to TPX2. Sequence conservation was limited to the putative spindle microtubule-associated region of TPX2, and intriguingly, D-TPX2 (Ssp1/Mei-38) lacks Aurora A- and kinesin-5-binding domains, which are highly conserved in other animal and plant species, including many insects such as ants and bees. D-TPX2 uniformly localised to kinetochore microtubule-enriched regions of the metaphase spindle in the S2 cell line, and it had microtubule binding and bundling activities in vitro. In comparison with other systems, the contribution of D-TPX2 to cell division seems to be minor; live cell imaging of microtubules and chromosomes after RNAi knockdown identified significant delay in chromosome congression in only 18% of the cells. Thus, while this conserved spindle protein is present in Drosophila, other mechanisms may largely compensate for its spindle assembly and chromosome segregation functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.