ArticleEukaryotic cell2012
Heterogeneous expression of the virulence-related adhesin Epa1 between individual cells and strains of the pathogen Candida glabrata.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Review
- Divergence in surface protein exposure between reference and clinical-derivedActa biochimica Polonica · 2026Article
- The transcription factor CgHaa1 plays a role in virulence of the pathogenic yeast Candida glabrata.FEMS yeast research · 2025Article
- Transposon-sequencing (Tn-seq) of the Candida glabrata reference strain CBS138 reveals epigenetic plasticity, structural variation, and intrinsic mechanisms of resistance to micafungin.G3 (Bethesda, Md.) · 2024Article
- The Candida glabrata Parent Strain Trap: How Phenotypic Diversity Affects Metabolic Fitness and Host Interactions.Microbiology spectrum · 2023Article
- Ploidy Variation and Spontaneous Haploid-Diploid Switching of Candida glabrata Clinical Isolates.mSphere · 2022Article
- Niacin Limitation PromotesPathogens (Basel, Switzerland) · 2022Article
- Host Age and Denture Wearing Jointly Contribute to Oral Colonization with Intrinsically Azole-Resistant Yeasts in the Elderly.Microorganisms · 2021Article
- Telomeric and Sub-Telomeric Structure and Implications in Fungal Opportunistic Pathogens.Microorganisms · 2021Review
- The Epithelial adhesin 1 tandem repeat region mediates protein display through multiple mechanisms.FEMS yeast research · 2020Article
- Divergent Approaches to Virulence inInternational journal of molecular sciences · 2019Review
- Adhesins inJournal of fungi (Basel, Switzerland) · 2018Review
- Relative Abundances of Candida albicans and Candida glabrata inApplied and environmental microbiology · 2018Article
- Heterologous Expression of a Novel Drug Transporter from the Malaria Parasite Alters Resistance to Quinoline Antimalarials.Scientific reports · 2018Article
- Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess.Cell chemical biology · 2017Article
- Candida glabrata Biofilms: How Far Have We Come?Journal of fungi (Basel, Switzerland) · 2017Review
- Article
- Hypothesis: Paralog Formation from Progenitor Proteins and Paralog Mutagenesis Spur the Rapid Evolution of Telomere Binding Proteins.Frontiers in genetics · 2016Article
- Structural Hot Spots Determine Functional Diversity of the Candida glabrata Epithelial Adhesin Family.The Journal of biological chemistry · 2015Article
- Article
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5 authors.
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Abstract
We investigated the relevance of gene expression heterogeneity to virulence properties of a major fungal pathogen, Candida glabrata. The organism's key virulence-associated factors include glycosylphosphatidylinositol-anchored adhesins, encoded subtelomerically by the EPA gene family. Individual-cell analyses of expression revealed very striking heterogeneity for Epa1, an adhesin that mediates ∼95% of adherence to epithelial cells in vitro. The heterogeneity in Epa1 was markedly greater than that known for other yeast genes. Sorted cells expressing high or low levels of Epa1 exhibited high and low adherence to epithelial cells, indicating a link between gene expression noise and potential virulence. The phenotypes of sorted subpopulations reverted to mixed phenotypes within a few generations. Variation in single-cell Epa1 protein and mRNA levels was correlated, consistent with transcriptional regulation of heterogeneity. Sir-dependent transcriptional silencing was the primary mechanism driving heterogeneous Epa1 expression in C. glabrata BG2, but not in CBS138 (ATCC 2001). Inefficient silencing in the latter strain was not due to a difference in EPA1 sequence or (sub)telomere length and was overcome by ectopic SIR3 expression. Moreover, differences between strains in the silencing dependence of EPA1 expression were evident across a range of clinical isolates, with heterogeneity being the greatest in strains where EPA1 was subject to silencing. The study shows how heterogeneity can impact the virulence-related properties of C. glabrata cell populations, with potential implications for microbial pathogenesis more broadly.
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