ArticleAmerican journal of physiology. Lung cellular and molecular physiology2012
Activation of calpains mediates early lung neutrophilic inflammation in ventilator-induced lung injury.
Article in American journal of physiology. Lung cellular and molecular physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- Mechanical ventilation activates PERK/eIF2α and NOD1 pathways that contribute to NF-κB-driven inflammation in VILI.Respiratory research · 2026Article
- Ventilator-Induced Lung Injury: Mechanotransduction and Potential Therapeutic Targets.MedComm · 2026Review
- ITLN1 exacerbates Crohn's colitis by driving ZBP1-dependent PANoptosis in intestinal epithelial cells through antagonizing TRIM8-mediated CAPN2 ubiquitination.International journal of biological sciences · 2025Article
- Cyclic stretch enhances neutrophil extracellular trap formation.BMC biology · 2024Article
- The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome.Biomedicines · 2023Article
- Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model.International journal of molecular sciences · 2022Article
- Effects of functionally diverse calpain system on immune cells.Immunologic research · 2021Review
- Enhancing Extracellular Adenosine Levels Restores Barrier Function in Acute Lung Injury Through Expression of Focal Adhesion Proteins.Frontiers in molecular biosciences · 2021Article
- Microarray Expression Profile and Analysis of Circular RNA Regulatory Network in Pulmonary Thromboembolism.International journal of general medicine · 2021Article
- Astragaloside IV attenuates inflammatory response mediated by NLRP-3/calpain-1 is involved in the development of pulmonary hypertension.Journal of cellular and molecular medicine · 2021Article
- In Vivo Calpain Knockdown Using Delivery of siRNA.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Effect and mechanism of calpains on pediatric lobar pneumonia.Bioengineered · 2017Article
- A Novel Cervical Spinal Cord Window Preparation Allows for Two-Photon Imaging of T-Cell Interactions with the Cervical Spinal Cord Microvasculature during Experimental Autoimmune Encephalomyelitis.Frontiers in immunology · 2017Article
- Redox-Dependent Calpain Signaling in Airway and Pulmonary Vascular Remodeling in COPD.Advances in experimental medicine and biology · 2017Article
- P120 regulates beta-catenin nuclear translocation through E-cadherin endocytosis in ventilator-induced lung injury.Oncotarget · 2016Article
- Critical role of calpain in inflammation.Biomedical reports · 2016Article
- Dual roles of calpain in facilitating Coxsackievirus B3 replication and prompting inflammation in acute myocarditis.International journal of cardiology · 2016Article
- The role of stretch-activated ion channels in acute respiratory distress syndrome: finally a new target?American journal of physiology. Lung cellular and molecular physiology · 2016Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Lung inflammatory responses in the absence of infection are considered to be one of primary mechanisms of ventilator-induced lung injury. Here, we determined the role of calpain in the pathogenesis of lung inflammation attributable to mechanical ventilation. Male C57BL/6J mice were subjected to high (28 ml/kg) tidal volume ventilation for 2 h in the absence and presence of calpain inhibitor I (10 mg/kg). To address the isoform-specific functions of calpain 1 and calpain 2 during mechanical ventilation, we utilized a liposome-based delivery system to introduce small interfering RNAs targeting each isoform in pulmonary vasculature in vivo. Mechanical ventilation with high tidal volume induced rapid (within minutes) and persistent calpain activation and lung inflammation as evidenced by neutrophil recruitment, production of TNF-α and IL-6, pulmonary vascular hyperpermeability, and lung edema formation. Pharmaceutical calpain inhibition significantly attenuated these inflammatory responses caused by lung hyperinflation. Depletion of calpain 1 or calpain 2 had a protective effect against ventilator-induced lung inflammatory responses. Inhibition of calpain activity by means of siRNA silencing or pharmacological inhibition also reduced endothelial nitric oxide (NO) synthase (NOS-3)-mediated NO production and subsequent ICAM-1 phosphorylation following high tidal volume ventilation. These results suggest that calpain activation mediates early lung inflammation during ventilator-induced lung injury via NOS-3/NO-dependent ICAM-1 phosphorylation and neutrophil recruitment. Inhibition of calpain activation may therefore provide a novel and promising strategy for the prevention and treatment of ventilator-induced lung injury.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.