ArticleBiotechnology and bioengineering2012
Rational bioprocess design for human pluripotent stem cell expansion and endoderm differentiation based on cellular dynamics.
Article in Biotechnology and bioengineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Decision Support Tools for Regenerative Medicine: Systematic Review.Journal of medical Internet research · 2018Pooled it
- Feeder-free generation of functional dendritic cells from human pluripotent stem cells.Journal for immunotherapy of cancer · 2026Article
- Analysis of the effects of bench-scale cell culture platforms and inoculum cell concentrations on PSC aggregate formation and culture.Frontiers in bioengineering and biotechnology · 2023Article
- Improved Differentiation of hESC-Derived Pancreatic Progenitors by Using Human Fetal Pancreatic Mesenchymal Cells in a Micro-scalable Three-Dimensional Co-culture System.Stem cell reviews and reports · 2022Article
- Automated optimization of endoderm differentiation on chip.Lab on a chip · 2021Article
- Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid.Biotechnology progress · 2021Article
- Review
- Article
- Mechanobiology of cells and cell systems, such as organoids.Biophysical reviews · 2019Review
- Bioprocess decision support tool for scalable manufacture of extracellular vesicles.Biotechnology and bioengineering · 2019Article
- Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications.Stem cells international · 2019Article
- Chemically controlled aggregation of pluripotent stem cells.Biotechnology and bioengineering · 2018Article
- Stem cell biomanufacturing under uncertainty: A case study in optimizing red blood cell production.AIChE journal. American Institute of Chemical Engineers · 2018Article
- Combinations of Activin A or Nicotinamide with the Pancreatic Transcription Factor PDX1 Support Differentiation of Human Amnion Epithelial Cells Toward a Pancreatic Lineage.Cellular reprogramming · 2017Article
- Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: considerations on process development.Scientific reports · 2017Article
- Climbing the mountain: experimental design for the efficient optimization of stem cell bioprocessing.Journal of biological engineering · 2017Review
- Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells.Journal of visualized experiments : JoVE · 2016Article
- Derivation of Endodermal Progenitors From Pluripotent Stem Cells.Journal of cellular physiology · 2015Review
- Human pluripotent stem cell-derived products: advances towards robust, scalable and cost-effective manufacturing strategies.Biotechnology journal · 2015Review
- Engineering three-dimensional stem cell morphogenesis for the development of tissue models and scalable regenerative therapeutics.Annals of biomedical engineering · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
We present a predictive bioprocess design strategy employing cell- and molecular-level analysis of rate-limiting steps in human pluripotent stem cell (hPSC) expansion and differentiation, and apply it to produce definitive endoderm (DE) progenitors using a scalable directed-differentiation technology. We define a bioprocess optimization parameter (L; targeted cell Loss) and, with quantitative cell division tracking and fate monitoring, identify and overcome key suspension bioprocess bottlenecks. Adapting process operating conditions to pivotal parameters (single cell survival and growth rate) in a cell-line-specific manner enabled adherent-equivalent expansion of hPSCs in feeder- and matrix-free defined-medium suspension culture. Predominantly instructive differentiation mechanisms were found to underlie a subsequent 18-fold expansion, during directed differentiation, to high-purity DE competent for further commitment along pancreatic and hepatic lineages. This study demonstrates that iPSC expansion and differentiation conditions can be prospectively specified to guide the enhanced production of target cells in a scale-free directed differentiation system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.