ArticleApplied and environmental microbiology2012
Survival of host-associated bacteroidales cells and their relationship with Enterococcus spp., Campylobacter jejuni, Salmonella enterica serovar Typhimurium, and adenovirus in freshwater microcosms as measured by propidium monoazide-quantitative PCR.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 94 citations in OpenAlex.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- Assessing the nucleic acid decay of human wastewater markers and enteric viruses in estuarine waters in Sydney, Australia.The Science of the total environment · 2024Article
- Comparing the decay of human wastewater-associated markers and enteric viruses in laboratory microcosms simulating estuarine waters in a temperate climatic zone using qPCR/RT-qPCR assays.The Science of the total environment · 2024Article
- Assessing the use of environmental DNA (eDNA) as a tool in the detection of human DNA in water.Journal of forensic sciences · 2022Article
- Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx-qPCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- The effects of removing dead bacteria by propidium monoazide on the profile of salivary microbiome.BMC oral health · 2021Article
- Escherichia coli Capacity to Repopulate Microcosms Under Osmotic/U.V. Synergic Stress in Tropical Waters.Current microbiology · 2021Article
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Decay of infectious adenovirus and coliphages in freshwater habitats is differentially affected by ambient sunlight and the presence of indigenous protozoa communities.Virology journal · 2020Article
- Spiking a Silty-Sand Reference Soil with Bacterial DNA: Limits and Pitfalls in the Discrimination of Live and Dead Cells When Applying Ethidium Monoazide (EMA) Treatment.Current microbiology · 2019Article
- Persistence and Decay of Fecal Microbiota in Aquatic Habitats.Microbiology and molecular biology reviews : MMBR · 2019Review
- Characterization of Salmonella Typhimurium and its monophasic variant 1,4, [5],12:i:- isolated from different sources.Folia microbiologica · 2019Article
- Comparison of pathogen-derived 'total risk' with indicator-based correlations for recreational (swimming) exposure.Environmental science and pollution research international · 2019Article
- Enhanced insights from human and animal host-associated molecular marker genes in a freshwater lake receiving wet weather overflows.Scientific reports · 2019Article
- Presence of bacteroidales as a predicator of human enteric viruses in Haihe River of Tianjin City, China.Environmental science and pollution research international · 2019Article
- Extended persistence of general and cattle-associated fecal indicators in marine and freshwater environment.The Science of the total environment · 2019Article
- Detection and Quantification of Viable but Non-culturableFrontiers in microbiology · 2019Article
- Relationships between Microbial Indicators and Pathogens in Recreational Water Settings.International journal of environmental research and public health · 2018Review
- Multi-tiered approach utilizing microbial source tracking and human associated-IMS/ATP for surveillance of human fecal contamination in Baja California, Mexico.The Science of the total environment · 2018Article
- Patterns of Host-Associated Fecal Indicators Driven by Hydrology, Precipitation, and Land Use Attributes in Great Lakes Watersheds.Environmental science & technology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ideal host-associated genetic fecal marker would be capable of predicting the presence of specific pathogens of concern. Flowthrough freshwater microcosms containing mixed feces and inocula of the pathogens Campylobacter jejuni, Salmonella enterica serovar Typhimurium, and adenovirus were placed at ambient temperature in the presence and absence of diurnal sunlight. The total Enterococcus DNA increased during the early periods (23 h) under sunlight exposure, even though cultivable Enterococcus and DNA in intact cells, as measured by propidium monoazide (PMA), decreased with first-order kinetics during the entire period. We found a significant difference in the decay of host-associated Bacteroidales cells between sunlight exposure and dark conditions (P value < 0.05), whereas the persistence of host-associated Bacteroidales DNA was comparable. The 2-log reduction times of adenovirus were 72 h for sunlight exposure and 99 h for dark conditions with similar decay rate constants (P value = 0.13). The persistences of fecal Bacteroidales cells and Campylobacter cells exposed to sunlight were similar, and host-associated Bacteroidales DNA and waterborne pathogen DNA were degraded at comparable rates (P values > 0.05). Overall, the ratio of quantitative PCR (qPCR) cycle threshold (C(T)) values with and without PMA treatment was indicative of the time elapsed since inoculation of the microcosm with (i) fecal material from different animal sources based on host-associated Bacteroidales and (ii) pure cultures of bacterial pathogens. The use of both PMA-qPCR and qPCR may yield more realistic information about recent sources of fecal contamination and result in improved prediction of waterborne pathogens and assessment of health risk.
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