ArticleApplied and environmental microbiology2012
Binding of human GII.4 norovirus virus-like particles to carbohydrates of romaine lettuce leaf cell wall materials.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Investigating the public health risks of low impact developments at residential, neighbourhood, and municipal levels.The Science of the total environment · 2020Pooled it
- Internalization and Localization of Murine Norovirus in Hydroponically Grown Green Onion (Allium Fistulosum L.).Food and environmental virology · 2026Article
- Structural Insights into the Interaction Between a Core-Fucosylated Foodborne Hexasaccharide (HViruses · 2026Article
- Use of polydimethylsiloxane topomimetic artificial blueberry, strawberry, and raspberry surfaces for the study of foodborne virus recovery and inactivation in berries.Frontiers in microbiology · 2026Article
- Human norovirus persists longer than Escherichia coli in sandy soil, independent of plant decaying materials.Scientific reports · 2025Article
- Evaluation of Porcine Gastric Mucin-Based Method for Extraction of Noroviruses from Seaweed Salad.Viruses · 2025Article
- The role of produce surface topography on foodborne virus inactivation on polydimethylsiloxane topomimetic artificial leaf surfaces and fresh leafy green surfaces.Frontiers in plant science · 2025Article
- Influence of pH, ion composition and carbohydrate recognition on the attachment ofCurrent research in parasitology & vector-borne diseases · 2025Article
- Common and Potential Emerging Foodborne Viruses: A Comprehensive Review.Life (Basel, Switzerland) · 2024Review
- Microgreen Variety Impacts Leaf Surface Persistence of a Human Norovirus Surrogate.Food and environmental virology · 2023Article
- Impact of irrigation water quality on human norovirus surrogate survival during leafy green production.Frontiers in plant science · 2023Article
- Assessment of Microbiological Quality of Fresh Vegetables and Oysters Produced in Buenos Aires Province, Argentina.Food and environmental virology · 2021Article
- Survival and Inactivation by Advanced Oxidative Process of Foodborne Viruses in Model Low-Moisture Foods.Food and environmental virology · 2021Article
- Effect of Direct Viral-Bacterial Interactions on the Removal of Norovirus From Lettuce.Frontiers in microbiology · 2021Article
- Hydrophobic Organic Matter Promotes Coxsackievirus B5 Stabilization and Protection from Heat.Food and environmental virology · 2020Article
- Article
- Specific Interactions between Human Norovirus and Environmental Matrices: Effects on the Virus Ecology.Viruses · 2019Review
- Tissue Distribution and Visualization of Internalized Human Norovirus in Leafy Greens.Applied and environmental microbiology · 2018Article
- Potential of Human Norovirus Surrogates andFrontiers in microbiology · 2018Article
- A Luciferase Immunoprecipitation System (LIPS) assay for profiling human norovirus antibodies.Journal of virological methods · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Norovirus (NoV) genogroup II genotype 4 (GII.4) strains are the dominant cause of the majority of food-borne outbreaks, including those that involve leafy greens, such as lettuce. Since human NoVs use carbohydrates of histo-blood group antigens as receptors/coreceptors, we examined the role of carbohydrates in the attachment of NoV to lettuce leaves by using virus-like particles (VLPs) of a human NoV/GII.4 strain. Immunofluorescence analysis showed that the VLPs attached to the leaf surface, especially to cut edges, stomata, and along minor veins. Binding was quantified using enzyme-linked immunosorbent assay (ELISA) performed on cell wall materials (CWM) from innermost younger leaves and outermost lamina of older leaves. The binding to CWM of older leaves was significantly (P < 0.05) higher (1.5- to 2-fold) than that to CWM of younger leaves. Disrupting the carbohydrates of CWM or porcine gastric mucin (PGM) (a carbohydrate control) using 100 mM sodium periodate (NaIO(4)) significantly decreased the binding an average of 17% in younger leaves, 43% in older leaves, and 92% for PGM. In addition, lectins recognizing GalNAc, GlcNAc, and sialic acid at 100 μg/ml significantly decreased the binding an average of 41%, 33%, and 20% on CWM of older leaves but had no effect on younger leaves. Lectins recognizing α-D-Gal, α-D-Man/α-D-Glc, and α-L-Fuc showed significant inhibition on CWM of older leaves as well as that of younger leaves. All lectins, except for the lectin recognizing α-D-Gal, significantly inhibited NoV VLP binding to PGM. Collectively, our results indicate that NoV VLPs bind to lettuce CWM by utilizing multiple carbohydrate moieties. This binding may enhance virus persistence on the leaf surface and prevent effective decontamination.
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