ArticleApplied and environmental microbiology2012
Transcriptome dynamics of Pseudomonas putida KT2440 under water stress.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection.Scientific reports · 2025Article
- From microbiome composition to functional engineering, one step at a time.Microbiology and molecular biology reviews : MMBR · 2023Review
- Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida.Environmental microbiology · 2023Article
- Transcriptional response of the xerotolerantFrontiers in microbiology · 2022Article
- Genome-wide gene expression changes of Pseudomonas veronii 1YdBTEX2 during bioaugmentation in polluted soils.Environmental microbiome · 2021Article
- Trehalose and α-glucan mediate distinct abiotic stress responses in Pseudomonas aeruginosa.PLoS genetics · 2021Article
- Alginate genes are required for optimal soil colonization and persistence byAccess microbiology · 2019Article
- Cultural, Transcriptomic, and Proteomic Analyses of Water-Stressed Cells of Actinobacterial Strains Isolated from Compost: Ecological Implications in the Fed-Batch Composting Process.Microbes and environments · 2016Article
- Mycelium-Like Networks Increase Bacterial Dispersal, Growth, and Biodegradation in a Model Ecosystem at Various Water Potentials.Applied and environmental microbiology · 2016Article
- Pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response.BMC microbiology · 2015Article
- Genes Associated with Desiccation and Osmotic Stress in Listeria monocytogenes as Revealed by Insertional Mutagenesis.Applied and environmental microbiology · 2015Article
- Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials.MicrobiologyOpen · 2014Article
- Identification of genes potentially involved in solute stress response in Sphingomonas wittichii RW1 by transposon mutant recovery.Frontiers in microbiology · 2014Article
- Gains of bacterial flagellar motility in a fungal world.Applied and environmental microbiology · 2013Article
- New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure.Microbial cell factories · 2013Article
- Cryotolerance strategies of Pseudomonads isolated from the rhizosphere of Himalayan plants.SpringerPlus · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water deprivation can be a major stressor to microbial life in surface and subsurface soil. In unsaturated soils, the matric potential (Ψ(m)) is often the main component of the water potential, which measures the thermodynamic availability of water. A low matric potential usually translates into water forming thin liquid films in the soil pores. Little is known of how bacteria respond to such conditions, where, in addition to facing water deprivation that might impair their metabolism, they have to adapt their dispersal strategy as swimming motility may be compromised. Using the pressurized porous surface model (PPSM), which allows creation of thin liquid films by controlling Ψ(m), we examined the transcriptome dynamics of Pseudomonas putida KT2440. We identified the differentially expressed genes in cells exposed to a mild matric stress (-0.4 MPa) for 4, 24, or 72 h. The major response was detected at 4 h before gradually disappearing. Upregulation of alginate genes was notable in this early response. Flagellar genes were not downregulated, and the microarray data even suggested increasing expression as the stress prolonged. Moreover, we tested the effect of polyethylene glycol 8000 (PEG 8000), a nonpermeating solute often used to simulate Ψ(m), on the gene expression profile and detected a different profile than that observed by directly imposing Ψ(m). This study is the first transcriptome profiling of KT2440 under directly controlled Ψ(m) and also the first to show the difference in gene expression profiles between a PEG 8000-simulated and a directly controlled Ψ(m).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.