ReviewAsian journal of andrology2012
Sperm preparation: state-of-the-art--physiological aspects and application of advanced sperm preparation methods.
Review in Asian journal of andrology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04962100 (Capacitation-associated Protein Tyrosine Phosphorylation As a Possible Biomarker of Sperm Selection in Sperm from Patients and Donors), which is not on this map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Capacitation-associated Protein Tyrosine Phosphorylation As a Possible Biomarker of Sperm Selection in Sperm from Patients and Donors
Who cites it
45 citing papers in PubMed, 142 citations in OpenAlex.
- A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT).Reproductive biology and endocrinology : RB&E · 2020Trial
- Epidemiology, Etiopathogenesis, Diagnosis, and Treatment of Male Infertility-Current Trends and Future Directions: A Narrative Review.Medicina (Kaunas, Lithuania) · 2026Review
- Sperm Preparation in ART: Traditional Methods and Emerging Technologies.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Comparison of Swim-Up and Microfluidic Sperm Sorting Methods in Selection of Sperm for Intracytoplasmic Sperm Injection.International journal of molecular sciences · 2025Article
- Deep Learning Models for Multi-Part Morphological Segmentation and Evaluation of Live Unstained Human Sperm.Sensors (Basel, Switzerland) · 2025Article
- A Narrative Review on the Sperm Selection Methods in Assisted Reproductive Technology: Out with the New, the Old Is Better?Journal of clinical medicine · 2025Review
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- Association between hypo-osmotic swelling test-induced tail swelling patterns and sperm quality.Future science OA · 2024Article
- Sperm Migration and Hyaluronic Acid Binding: Implications for Male Fertility Evaluation.International journal of molecular sciences · 2024Article
- Assessing the Impact of the Novel Sperm Selection Technique 'Annexin-V Coated Polystyrene Bead Technique' on Mouse Assisted Reproductive Techniques Outcomes: Preliminary Findings.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Timing of semen cryopreservation: before or after processing?Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia · 2024Article
- The Impact of a Very Short Abstinence Period on Assisted Reproductive Technique Outcomes: A Systematic Review and Meta-Analysis.Antioxidants (Basel, Switzerland) · 2023Review
- Isolating motile sperm cell sorting using biocompatible electrospun membranes.Scientific reports · 2022Article
- Article
- Action of swim-up and caffeine on equine frozen sperm.Animal reproduction · 2022Article
- Correlations between elevated basal sperm DNA fragmentation and the clinical outcomes in women undergoing IUI.Frontiers in endocrinology · 2022Article
- PICSI vs. MACS for abnormal sperm DNA fragmentation ICSI cases: a prospective randomized trial.Journal of assisted reproduction and genetics · 2020Article
- High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition.Scientific reports · 2020Article
- Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential.International journal of fertility & sterility · 2020Article
- Hitting the wall: Human sperm velocity recovery under ultra-confined conditions.Biomicrofluidics · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For assisted reproduction technologies (ART), numerous techniques were developed to isolate spermatozoa capable of fertilizing oocytes. While early methodologies only focused on isolating viable, motile spermatozoa, with progress of ART, particularly intracytoplasmic sperm injection (ICSI), it became clear that these parameters are insufficient for the identification of the most suitable spermatozoon for fertilization. Conventional sperm preparation techniques, namely, swim-up, density gradient centrifugation and glass wool filtration, are not efficient enough to produce sperm populations free of DNA damage, because these techniques are not physiological and not modeled on the stringent sperm selection processes taking place in the female genital tract. These processes only allow one male germ cell out of tens of millions to fuse with the oocyte. Sites of sperm selection in the female genital tract are the cervix, uterus, uterotubal junction, oviduct, cumulus oophorus and the zona pellucida. Newer strategies of sperm preparation are founded on: (i) morphological assessment by means of 'motile sperm organelle morphological examination (MSOME)'; (ii) electrical charge; and (iii) molecular binding characteristics of the sperm cell. Whereas separation methods based on electrical charge take advantage of the sperm's adherence to a test tube surface or separate in an electrophoresis, molecular binding techniques use Annexin V or hyaluronic acid (HA) as substrates. Techniques in this category are magnet-activated cell sorting, Annexin V-activated glass wool filtration, flow cytometry and picked spermatozoa for ICSI (PICSI) from HA-coated dishes and HA-containing media. Future developments may include Raman microspectrometry, confocal light absorption and scattering spectroscopic microscopy and polarization microscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.