ArticleThe Plant cell2011
Maize rough endosperm3 encodes an RNA splicing factor required for endosperm cell differentiation and has a nonautonomous effect on embryo development.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- The mysterious dialogue between the embryo, endosperm, and seed coat, and its implications for seed traits.Journal of experimental botany · 2026Review
- Genomic basis underlying alternative transcripts-mediated drought tolerance in maize.Genome biology · 2026Article
- From development to yield: genetic and molecular regulation of agronomic traits in maize seeds.Frontiers in plant science · 2026Review
- Pre-mRNA Splicing Functions in Plant Sexual Reproduction Development.Plants (Basel, Switzerland) · 2025Review
- Genetic analysis of human RNA binding motif protein 48 (RBM48) reveals an essential role in U12-type intron splicing.Genetics · 2022Article
- Paternal imprinting of dosage-effect defective1 contributes to seed weight xenia in maize.Nature communications · 2022Article
- Post-transcriptional regulation of seed dormancy and germination: Current understanding and future directions.Plant communications · 2021Review
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing.Rice (New York, N.Y.) · 2020Article
- Embryo-Endosperm Interaction and Its Agronomic Relevance to Rice Quality.Frontiers in plant science · 2020Review
- Intra-Kernel Reallocation of Proteins in Maize Depends on VP1-Mediated Scutellum Development and Nutrient Assimilation.The Plant cell · 2019Article
- The nuclear-localized PPR protein OsNPPR1 is important for mitochondrial function and endosperm development in rice.Journal of experimental botany · 2019Article
- The complexity of alternative splicing and landscape of tissue-specific expression in lotus (Nelumbo nucifera) unveiled by Illumina- and single-molecule real-time-based RNA-sequencing.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- RNA Binding Motif Protein 48 Is Required for U12 Splicing and Maize Endosperm Differentiation.The Plant cell · 2019Article
- Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane.Frontiers in plant science · 2019Article
- Alternative Splicing Regulation During Light-Induced Germination ofFrontiers in plant science · 2019Article
- Ovule Gene Expression Analysis in Sexual and Aposporous ApomicticFrontiers in plant science · 2019Article
- Carbohydrate reserves and seed development: an overview.Plant reproduction · 2018Review
- Alternative Splicing as a Regulator of Early Plant Development.Frontiers in plant science · 2018Review
- Genomic Imprinting Was Evolutionarily Conserved during Wheat Polyploidization.The Plant cell · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endosperm and embryo development are coordinated via epigenetic regulation and signaling between these tissues. In maize (Zea mays), the endosperm-embryo signals are not known, but endosperm cellularization is a key event for embryos to form shoots and roots. We screened seed mutants for nonautonomous functions in endosperm and embryo development with genetically nonconcordant seeds and identified the recessive mutant rough endosperm3 (rgh3). The wild-type Rgh3 allele is required in the endosperm for embryos to develop and has an autonomous role in embryo and seedling development. Endosperm cell differentiation is defective in rgh3. Results from endosperm cell culture indicate that rgh3 mutants remain in a proliferative state through mid-seed development. Rgh3 encodes the maize U2AF(35) Related Protein (URP), an RNA splicing factor involved in both U2 and U12 splicing. The Rgh3 allele produces at least 19 alternative splice variants with only one isoform encoding a full-length ortholog to URP. The full-length RGH3α isoform localizes to the nucleolus and displays a speckled pattern within the nucleoplasm, and RGH3α colocalizes with U2AF(65). A survey of alternatively spliced transcripts found that, in the rgh3 mutant, a fraction of noncanonical splicing events are altered. Our findings suggest that differentiation of maize endosperm cell types is necessary for embryos to develop. The molecular cloning of Rgh3 suggests that alternative RNA splicing is needed for cell differentiation, development, and plant viability.
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