ArticleAtherosclerosis2012
Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy.
Article in Atherosclerosis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Dual-modality fluorescence lifetime imaging-optical coherence tomography intravascular catheter system with freeform catheter optics.Journal of biomedical optics · 2022Article
- Spectral and lifetime resolution of fundus autofluorescence in advanced age-related macular degeneration revealing different signal sources.Acta ophthalmologica · 2022Article
- Comprehensive Assessment of High-Risk Plaques by Dual-Modal Imaging Catheter in Coronary Artery.JACC. Basic to translational science · 2021Article
- Label-Free Visualization and Quantification of Biochemical Markers of Atherosclerotic Plaque Progression Using Intravascular Fluorescence Lifetime.JACC. Cardiovascular imaging · 2021Article
- Blind deconvolution estimation by multi-exponential models and alternated least squares approximations: Free-form and sparse approach.PloS one · 2021Article
- Fluorescence Lifetimes and Spectra of RPE and Sub-RPE Deposits in Histology of Control and AMD Eyes.Investigative ophthalmology & visual science · 2020Article
- Dual-modality optical coherence tomography and frequency-domain fluorescence lifetime imaging microscope system for intravascular imaging.Journal of biomedical optics · 2020Article
- Direct frequency domain fluorescence lifetime imaging using field programmable gate arrays for real time processing.The Review of scientific instruments · 2020Article
- Investigating Origins of FLIm Contrast in Atherosclerotic Lesions Using Combined FLIm-Raman Spectroscopy.Frontiers in cardiovascular medicine · 2020Article
- Comprehensive intravascular imaging of atherosclerotic plaque in vivo using optical coherence tomography and fluorescence lifetime imaging.Scientific reports · 2018Article
- Real-time visualization of two-photon fluorescence lifetime imaging microscopy using a wavelength-tunable femtosecond pulsed laser.Biomedical optics express · 2018Article
- Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography.Biomedical optics express · 2018Article
- Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence.Biomedical optics express · 2016Article
- Lensless, ultra-wideband fiber optic rotary joint for biomedical applications.Optics letters · 2016Article
- Deconvolution of fluorescence lifetime imaging microscopy by a library of exponentials.Optics express · 2015Article
- Simultaneous morphological and biochemical endogenous optical imaging of atherosclerosis.European heart journal. Cardiovascular Imaging · 2015Article
- Blind deconvolution estimation of fluorescence measurements through quadratic programming.Journal of biomedical optics · 2015Article
- Extended output phasor representation of multi-spectral fluorescence lifetime imaging microscopy.Biomedical optics express · 2015Article
- Blind source separation of ex-vivo aorta tissue multispectral images.Biomedical optics express · 2015Article
- Application of time-resolved autofluorescence to label-free in vivo optical mapping of changes in tissue matrix and metabolism associated with myocardial infarction and heart failure.Biomedical optics express · 2015Article
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6 authors.
Funding
Abstract
objectiveTo investigate the potential of endogenous multispectral fluorescence lifetime imaging microscopy (FLIM) for biochemical characterization of human coronary atherosclerotic plaques.
methodsEndogenous multispectral FLIM imaging was performed on the lumen of 58 segments of postmortem human coronary artery. The fluorescence was separated into three emission bands targeting the three main arterial endogenous fluorophores (390±20 nm for collagen, 452±22.5 nm for elastin, and 550±20 for lipids). The fluorescence normalized intensity and average lifetime from each emission band was used to classify each pixel of an image as either "High-Collagen", "High-Lipids" or "Low-Collagen/Lipids" via multiclass Fisher's linear discriminant analysis.
resultsClassification of plaques as either "High-Collagen", "High-Lipids" or "Low-Collagen/Lipids" based on the endogenous multispectral FLIM was achieved with a sensitivity/specificity of 96/98%, 89/99%, and 99/99%, respectively, where histopathology served as the gold standard.
conclusionThe endogenous multispectral FLIM approach we have taken, which can readily be adapted for in vivo intravascular catheter based imaging, is capable of reliably identifying plaques with high content of either collagen or lipids.
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