ArticleInternational journal for parasitology2012
A method for rapid regulation of protein expression in Trypanosoma cruzi.
Article in International journal for parasitology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- A limitation lifted: A conditional knockdown system reveals essential roles for Polo-like kinase and Aurora kinase 1 inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Conditional Protein Degradation System To Study Essential Gene Function in Cryptosporidium parvum.mBio · 2020Article
- Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48.Molecular medicine reports · 2018Article
- Kinases as druggable targets in trypanosomatid protozoan parasites.Chemical reviews · 2014Review
- Destabilization domain approach adapted for regulated protein expression in the protozoan parasite Entamoeba histolytica.International journal for parasitology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.