ArticleJournal of proteome research2012
Mapping N-linked glycosylation sites in the secretome and whole cells of Aspergillus niger using hydrazide chemistry and mass spectrometry.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 68 citations in OpenAlex.
- Integrative perspectives on glycosylation networks in fungi and oomycetes.Journal of microbiology (Seoul, Korea) · 2025Review
- Systematic characterization of extracellular glycoproteins using mass spectrometry.Mass spectrometry reviews · 2023Review
- Different Schiff Bases-Structure, Importance and Classification.Molecules (Basel, Switzerland) · 2022Review
- [Recent advances in functionalized magnetic nanomaterials for glycoprotein and glycopeptide enrichment].Se pu = Chinese journal of chromatography · 2021Review
- Importance of evaluating protein glycosylation in pluripotent stem cell-derived cardiomyocytes for research and clinical applications.Pflugers Archiv : European journal of physiology · 2021Review
- Biochemical properties of a native β-1,4-mannanase fromBiochemistry and biophysics reports · 2021Article
- Global and site-specific analysis of protein glycosylation in complex biological systems with Mass Spectrometry.Mass spectrometry reviews · 2019Review
- Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species.Scientific reports · 2018Article
- A community-driven reconstruction of theFungal biology and biotechnology · 2018Article
- Simultaneous Quantitation of Glycoprotein Degradation and Synthesis Rates by Integrating Isotope Labeling, Chemical Enrichment, and Multiplexed Proteomics.Analytical chemistry · 2017Article
- An efficient method for native protein purification in the selected range from prostate cancer tissue digests.Chinese clinical oncology · 2016Article
- Intact glycopeptide characterization using mass spectrometry.Expert review of proteomics · 2016Review
- In-depth analysis of secretome and N-glycosecretome of human hepatocellular carcinoma metastatic cell lines shed light on metastasis correlated proteins.Oncotarget · 2016Article
- Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.Biotechnology for biofuels · 2016Article
- Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells.Proteomes · 2015Review
- Structural analysis of N- and O-glycans using ZIC-HILIC/dialysis coupled to NMR detection.Fungal genetics and biology : FG & B · 2014Article
- MS-GF+ makes progress towards a universal database search tool for proteomics.Nature communications · 2014Article
- A universal chemical enrichment method for mapping the yeast N-glycoproteome by mass spectrometry (MS).Molecular & cellular proteomics : MCP · 2014Article
- Characterization of intact N- and O-linked glycopeptides using higher energy collisional dissociation.Analytical biochemistry · 2014Article
- Mass spectrometry-based N-glycoproteomics for cancer biomarker discovery.Clinical proteomics · 2014Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Protein glycosylation (e.g., N-linked glycosylation) is known to play an essential role in both cellular functions and secretory pathways; however, our knowledge of in vivo N-glycosylated sites is very limited for the majority of fungal organisms including Aspergillus niger. Herein, we present the first extensive mapping of N-glycosylated sites in A. niger by applying an optimized solid phase glycopeptide enrichment protocol using hydrazide-modified magnetic beads. The enrichment protocol was initially optimized using both mouse blood plasma and A. niger secretome samples, and it was demonstrated that the protein-level enrichment protocol offered superior performance over the peptide-level protocol. The optimized protocol was then applied to profile N-glycosylated sites from both the secretome and whole cell lysates of A. niger. A total of 847 N-glycosylated sites from 330 N-glycoproteins (156 proteins from the secretome and 279 proteins from whole cells) were confidently identified by LC-MS/MS. The identified N-glycoproteins in the whole cell lysate were primarily localized in the plasma membrane, endoplasmic reticulum, Golgi apparatus, lysosome, and storage vacuoles, supporting the important role of N-glycosylation in the secretory pathways. In addition, these glycoproteins are involved in many biological processes including gene regulation, signal transduction, protein folding and assembly, protein modification, and carbohydrate metabolism. The extensive coverage of N-glycosylated sites and the observation of partial glycan occupancy on specific sites in a number of enzymes provide important initial information for functional studies of N-linked glycosylation and their biotechnological applications in A. niger.
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