ArticleProceedings of the National Academy of Sciences of the United States of America2011
Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 134 papers.
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134 citing papers in PubMed.
- Pectin under stress: Pectin methylesterases at the core of cell wall remodeling, integrity sensing, and signaling in plants.Plant communications · 2026Review
- Genetic control of tomato fruit cracking through SlPMEI27-dependent pectin modification.Molecular horticulture · 2026Article
- Wall-associated kinase-like4 regulates plant salt tolerance by maintaining cell wall pectin homeostasis and cellular redox homeostasis in Arabidopsis.Plant molecular biology · 2026Article
- A characterization of recombinant Arabidopsis FRIABLE1 (FRB1) reveals robust rhamnogalacturonan-I rhamnosyltransferase activity and critical catalytic residues.The Journal of biological chemistry · 2026Article
- Genome-Wide Identification and Characterization of the MaizePlants (Basel, Switzerland) · 2026Article
- Gibberellic acid (GA) alleviates Al toxicity in rice (Oryza sativa) through a nitric oxide (NO) dependent pathway.Planta · 2026Article
- Changes in Cell Aggregation inPlants (Basel, Switzerland) · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- Transcriptomic profiles reveal hormonal regulation of sugar-induced stolon initiation in potato.Scientific reports · 2025Article
- ROS homeostasis and cell wall biosynthesis pathway are involved in female inflorescence development of birch (Betula platyphylla).BMC plant biology · 2025Article
- Gibberellin signaling regulates pectin biosynthesis in Arabidopsis.Nature communications · 2025Article
- Potential of Bio-Sourced Oligogalacturonides in Crop Protection.Molecules (Basel, Switzerland) · 2025Review
- Multiprotein Complexes of Plant Glycosyltransferases Involved in Their Function and Trafficking.Plants (Basel, Switzerland) · 2025Review
- A Fresh Look at Celery Collenchyma and Parenchyma Cell Walls Through a Combination of Biochemical, Histochemical, and Transcriptomic Analyses.International journal of molecular sciences · 2025Article
- Enzyme Repertoires and Genomic Insights into Lycium barbarum Pectin Polysaccharide Biosynthesis.Genomics, proteomics & bioinformatics · 2025Article
- Impact ofBiotech (Basel (Switzerland)) · 2025Article
- Integrative dynamics of cell wall architecture and plant growth under salt stress.Frontiers in plant science · 2025Review
- Exocyst subunits EXO70B1 and B2 contribute to stomatal dynamics and cell wall modifications.Frontiers in plant science · 2025Article
- Heterogeneity in Mechanical Properties of Plant Cell Walls.Plants (Basel, Switzerland) · 2024Review
74 more citing papers are in PubMed but not listed here.
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9 authors.
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Abstract
Plant cell wall pectic polysaccharides are arguably the most complex carbohydrates in nature. Progress in understanding pectin synthesis has been slow due to its complex structure and difficulties in purifying and expressing the low-abundance, Golgi membrane-bound pectin biosynthetic enzymes. Arabidopsis galacturonosyltransferase (GAUT) 1 is an α-1,4-galacturonosyltransferase (GalAT) that synthesizes homogalacturonan (HG), the most abundant pectic polysaccharide. We now show that GAUT1 functions in a protein complex with the homologous GAUT7. Surprisingly, although both GAUT1 and GAUT7 are type II membrane proteins with single N-terminal transmembrane-spanning domains, the N-terminal region of GAUT1, including the transmembrane domain, is cleaved in vivo. This raises the question of how the processed GAUT1 is retained in the Golgi, the site of HG biosynthesis. We show that the anchoring of GAUT1 in the Golgi requires association with GAUT7 to form the GAUT1:GAUT7 complex. Proteomics analyses also identified 12 additional proteins that immunoprecipitate with the GAUT1:GAUT7 complex. This study provides conclusive evidence that the GAUT1:GAUT7 complex is the catalytic core of an HG:GalAT complex and that cell wall matrix polysaccharide biosynthesis occurs via protein complexes. The processing of GAUT1 to remove its N-terminal transmembrane domain and its anchoring in the Golgi by association with GAUT7 provides an example of how specific catalytic domains of plant cell wall biosynthetic glycosyltransferases could be assembled into protein complexes to enable the synthesis of the complex and developmentally and environmentally plastic plant cell wall.
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