ArticleCirculation research2012
Etsrp/Etv2 is directly regulated by Foxc1a/b in the zebrafish angioblast.
Article in Circulation research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 62 citations in OpenAlex.
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- BCL6B-dependent suppression of ETV2 hampers endothelial cell differentiation.Stem cell research & therapy · 2024Article
- Multiple cis-regulatory elements control prox1a expression in distinct lymphatic vascular beds.Development (Cambridge, England) · 2024Article
- Article
- Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development-New Insights From Technical Advances.Frontiers in cell and developmental biology · 2022Review
- The regulatory role of pioneer factors during cardiovascular lineage specification - A mini review.Frontiers in cardiovascular medicine · 2022Review
- Article
- Gfi1aa/Lsd1 Facilitates Hemangioblast Differentiation Into Primitive Erythrocytes by TargetingFrontiers in cell and developmental biology · 2021Article
- Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle.Nature communications · 2020Article
- Embryonic ethanol exposure alters expression of sox2 and other early transcripts in zebrafish, producing gastrulation defects.Scientific reports · 2020Article
- Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells.Frontiers in cell and developmental biology · 2020Article
- ETV2/ER71 regulates the generation of FLK1Stem cell research & therapy · 2019Article
- In vivo transduction of ETV2 improves cardiac function and induces vascular regeneration following myocardial infarction.Experimental & molecular medicine · 2019Article
- ETV2/ER71 Transcription Factor as a Therapeutic Vehicle for Cardiovascular Disease.Theranostics · 2019Review
- ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression.Developmental biology · 2018Article
- Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach.BMC developmental biology · 2018Article
- The transcription factor Foxc1a in zebrafish directly regulates expression ofThe Journal of biological chemistry · 2018Article
- Re-evaluating the functional landscape of the cardiovascular system during development.Biology open · 2017Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
rationaleEndothelial cells are developmentally derived from angioblasts specified in the mesodermal germ cell layer. The transcription factor etsrp/etv2 is at the top of the known genetic hierarchy for angioblast development. The transcriptional events that induce etsrp expression and angioblast specification are not well understood.
objectiveWe generated etsrp:gfp transgenic zebrafish and used them to identify regulatory regions and transcription factors critical for etsrp expression and angioblast specification from mesoderm. METHODS AND
resultsTo investigate the mechanisms that initiate angioblast cell transcription during embryogenesis, we have performed promoter analysis of the etsrp locus in zebrafish. We describe three enhancer elements sufficient for endothelial gene expression when place in front of a heterologous promoter. The deletion of all 3 regulatory regions led to a near complete loss of endothelial expression from the etsrp promoter. One of the enhancers, located 2.3 kb upstream of etsrp contains a consensus FOX binding site that binds Foxc1a and Foxc1b in vitro by EMSA and in vivo using ChIP. Combined knockdown of foxc1a/b, using morpholinos, led to a significant decrease in etsrp expression at early developmental stages as measured by quantitative reverse transcriptase-polymerase chain reaction and in situ hybridization. Decreased expression of primitive erythrocyte genes scl and gata1 was also observed, whereas pronephric gene pax2a was relatively normal in expression level and pattern.
conclusionsThese findings identify mesodermal foxc1a/b as a direct upstream regulator of etsrp in angioblasts. This establishes a new molecular link in the process of mesoderm specification into angioblast.
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