ArticleJournal of cell science2011
Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.
Article in Journal of cell science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling.Communications biology · 2024Article
- Article
- Transcription factors SltA and CrzA reversely regulate calcium homeostasis under calcium-limited conditions.Applied and environmental microbiology · 2023Article
- Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Component Interaction of ESCRT Complexes Is Essential for Endocytosis-Dependent Growth, Reproduction, DON Production and Full Virulence inFrontiers in microbiology · 2019Article
- Article
- Mutational analysis of the Aspergillus ambient pH receptor PalH underscores its potential as a target for antifungal compounds.Molecular microbiology · 2016Article
- Proteolytic activation of both components of the cation stress-responsive Slt pathway in Aspergillus nidulans.Molecular biology of the cell · 2016Article
- Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: a white paper.Fungal biology and biotechnology · 2016Article
- Article
- Molecular Components of the Neurospora crassa pH Signaling Pathway and Their Regulation by pH and the PAC-3 Transcription Factor.PloS one · 2016Article
- Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein.Molecular microbiology · 2015Article
- A second component of the SltA-dependent cation tolerance pathway in Aspergillus nidulans.Fungal genetics and biology : FG & B · 2015Article
- Fast-suppressor screening for new components in protein trafficking, organelle biogenesis and silencing pathway in Arabidopsis thaliana using DEX-inducible FREE1-RNAi plants.Journal of genetics and genomics = Yi chuan xue bao · 2015Article
- Aspergillus nidulans Ambient pH Signaling Does Not Require Endocytosis.Eukaryotic cell · 2015Article
- Survey of protein-DNA interactions in Aspergillus oryzae on a genomic scale.Nucleic acids research · 2015Article
- The Cryptococcus neoformans alkaline response pathway: identification of a novel rim pathway activator.PLoS genetics · 2015Article
- Signaling events of the Rim101 pathway occur at the plasma membrane in a ubiquitination-dependent manner.Molecular and cellular biology · 2014Article
- Identification of interphase functions for the NIMA kinase involving microtubules and the ESCRT pathway.PLoS genetics · 2014Article
- Live-cell imaging of Aspergillus nidulans autophagy: RAB1 dependence, Golgi independence and ER involvement.Autophagy · 2013Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Aspergillus pal pathway hijacks ESCRT proteins into ambient pH signalling complexes. We show that components of ESCRT-0, ESCRT-I, ESCRT-II and ESCRT-III are nearly essential for growth, precluding assessment of null mutants for pH signalling or trafficking. This severely debilitating effect is rescued by loss-of-function mutations in two cation tolerance genes, one of which, sltA, encodes a transcription factor whose inactivation promotes hypervacuolation. Exploiting a conditional expression sltA allele, we demonstrate that deletion of vps27 (ESCRT-0), vps23 (ESCRT-I), vps36 (ESCRT-II), or vps20 or vps32 (both ESCRT-III) leads to numerous small vacuoles, a phenotype also suppressed by SltA downregulation. This situation contrasts with normal vacuoles and vacuole-associated class E compartments seen in Saccharomyces cerevisiae ESCRT null mutants. Exploiting the suppressor phenotype of sltA(-) mutations, we establish that Vps23, Vps36, Vps20 and Vps32 are essential for pH signalling. Phosphatidylinositol 3-phosphate-recognising protein Vps27 (ESCRT-0) is not, consistent with normal pH signalling in rabB null mutants unable to recruit Vps34 kinase to early endosomes. In contrast to the lack of pH signalling in the absence of Vps20 or Vps32, detectable signalling occurs in the absence of ESCRT-III subunit Vps24. Our data support a model in which certain ESCRT proteins are recruited to the plasma membrane to mediate pH signalling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.