ArticleThe Journal of biological chemistry2012
Two-partner secretion of gram-negative bacteria: a single β-barrel protein enables transport across the outer membrane.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Construction and characterization of a nanopore derived from the transmembrane domain of a trimeric autotransporter adhesin.Frontiers in bioengineering and biotechnology · 2026Article
- Article
- Large-Scale Conformational Changes of FhaC Provide Insights Into the Two-Partner Secretion Mechanism.Frontiers in molecular biosciences · 2022Article
- Folding Control in the Path of Type 5 Secretion.Toxins · 2021Review
- Review
- Bordetella adenylate cyclase toxin interacts with filamentous haemagglutinin to inhibit biofilm formation in vitro.Molecular microbiology · 2017Article
- Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions.Frontiers in cellular and infection microbiology · 2017Review
- Slam is an outer membrane protein that is required for the surface display of lipidated virulence factors in Neisseria.Nature microbiology · 2016Article
- Outer membrane protein biogenesis in Gram-negative bacteria.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Conserved Omp85 lid-lock structure and substrate recognition in FhaC.Nature communications · 2015Article
- Structure-function analysis reveals that the Pseudomonas aeruginosa Tps4 two-partner secretion system is involved in CupB5 translocation.Protein science : a publication of the Protein Society · 2015Article
- TtOmp85, a β-barrel assembly protein, functions by barrel augmentation.Biochemistry · 2015Article
- FhaC takes a bow to FHA in the two-partner do-si-do.Molecular microbiology · 2014Article
- Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio.PLoS genetics · 2014Article
- The structural basis of autotransporter translocation by TamA.Nature structural & molecular biology · 2013Article
- Article
- The Burkholderia bcpAIOB genes define unique classes of two-partner secretion and contact dependent growth inhibition systems.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms of protein secretion by pathogenic bacteria remain poorly understood. In gram-negative bacteria, the two-partner secretion pathway exports large, mostly virulence-related "TpsA" proteins across the outer membrane via their dedicated "TpsB" transporters. TpsB transporters belong to the ubiquitous Omp85 superfamily, whose members are involved in protein translocation across, or integration into, cellular membranes. The filamentous hemagglutinin/FhaC pair of Bordetella pertussis is a model two-partner secretion system. We have reconstituted the TpsB transporter FhaC into proteoliposomes and demonstrate that FhaC is the sole outer membrane protein required for translocation of its cognate TpsA protein. This is the first in vitro system for analyzing protein secretion across the outer membrane of gram-negative bacteria. Our data also provide clear evidence for the protein translocation function of Omp85 transporters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.