ArticleBiosensors & bioelectronics2012
Visual detection of gene mutations based on isothermal strand-displacement polymerase reaction and lateral flow strip.
Article in Biosensors & bioelectronics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 61 citations in OpenAlex.
- Protein-Based Anchoring Methods for Nucleic Acid Detection in Lateral Flow Format Assays.Micromachines · 2023Article
- Paper-based biosensors as point-of-care diagnostic devices for the detection of cancers: a review of innovative techniques and clinical applications.Frontiers in oncology · 2023Review
- Recent advances and challenges of biosensing in point-of-care molecular diagnosis.Sensors and actuators. B, Chemical · 2021Review
- Rapid visual genotyping method for germline mutants with small genomic fragment deletion by allele-specific PCR and lateral flow nucleic acid biosensor.Molecular biology reports · 2021Article
- Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy.Beilstein journal of nanotechnology · 2020Review
- Lateral flow biosensors based on the use of micro- and nanomaterials: a review on recent developments.Mikrochimica acta · 2019Review
- A lateral flow assay for copper(II) utilizing catalytic and stem-loop based signal amplification.Mikrochimica acta · 2019Article
- A review on microscale polymerase chain reaction based methods in molecular diagnosis, and future prospects for the fabrication of fully integrated portable biomedical devices.Mikrochimica acta · 2018Review
- [Application of gold nanoparticles-based lateral flow strip for dengue virus detection based on strand-displacement amplification].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2018Article
- Lateral flow test for visual detection of silver (I) based on cytosine-Ag(I)-cytosine interaction in C-rich oligonucleotides.Mikrochimica acta · 2017Article
- Visual detection of glial cell line-derived neurotrophic factor based on a molecular translator and isothermal strand-displacement polymerization reaction.Drug design, development and therapy · 2015Article
- Simultaneous detection of nucleic acid and protein using gold nanoparticles and lateral flow device.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2014Article
- Diagnostic devices for isothermal nucleic acid amplification.Sensors (Basel, Switzerland) · 2012Review
- A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.Biotechnology advancesReview
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Here, we describe a simple and sensitive approach for visual detection of gene mutations based on isothermal strand-displacement polymerase reactions (ISDPR) and lateral flow strip (LFS). The concept was first demonstrated by detecting the R156H-mutant gene of keratin 10 in Epidermolytic hyperkeratosis (EHK). In the presence of biotin-modified hairpin DNA and digoxin-modified primer, the R156H-mutant DNA triggered the ISDPR to produce numerous digoxin- and biotin-attached duplex DNA products. The product was detected on the LFS through dual immunoreactions (anti-digoxin antibody on the gold nanoparticle (Au-NP) and digoxin on the duplex, anti-biotin antibody on the LFS test zone and biotin on the duplex). The accumulation of Au-NPs produced the characteristic red band, enabling visual detection of the mutant gene without instrumentation. After systematic optimization of the ISDPR experimental conditions and the parameters of the assay, the current approach was capable of detecting as low as 1-fM R156H-mutant DNA within 75 min without instrumentation. Differentiation of R156H- and R156C-mutant DNA on the R156 mutation site was realized by using fluorescein- and biotin-modified hairpin probes in the ISDPR process. The approach thus provides a simple, sensitive, and low-cost tool for the detection of gene mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.