ArticlePlant methods2011
Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Herbicide resistance in grass weeds: Epigenetic regulation matters too.Frontiers in plant science · 2022Review
- Effect of Salt Stress on Cytosine Methylation within GL2, AnInternational journal of molecular sciences · 2019Article
- Genome-Wide DNA Methylation Comparison betweenInternational journal of molecular sciences · 2018Article
- Altered Transcription and Neofunctionalization of Duplicated Genes Rescue the Harmful Effects of a Chimeric Gene inThe Plant cell · 2016Article
- Widespread and evolutionary analysis of a MITE family Monkey King in Brassicaceae.BMC plant biology · 2015Article
- Targeted DNA methylation analysis by next-generation sequencing.Journal of visualized experiments : JoVE · 2015Article
- Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa.Applications in plant sciences · 2014Article
- Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba.The Plant cell · 2014Article
- Comparative analysis of FLC homologues in Brassicaceae provides insight into their role in the evolution of oilseed rape.PloS one · 2012Article
- Promoter variation and transcript divergence in Brassicaceae lineages of FLOWERING LOCUS T.PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Accurate analysis of DNA methylation by bisulphite sequencing depends on the complete conversion of all cytosines into uracil. Until now there has been no standard or universal gene identified as an endogenous control to monitor the conversion frequency in plants. Here, we report the development of PCR based assays for one nuclear gene IND (INDEHISCENT) and two mitochondrial genes, NAD (NICOTINAMIDE ADENINE DINUCLEOTIDE) and ATP1 (ATPase SUBUNIT 1). We demonstrated their efficacy as bisulphite conversion controls in Brassica and other plant taxa. The target regions amplified by four primer pairs were found to be consistently free from DNA methylation. Primer pairs for IND.a and NAD were effective within Brassica species, whereas two primer pairs for ATP1 provided reliable controls across a representative range of dicot and monocot angiosperm species. These primer sets may therefore be adopted as controls in plant methylation analysis for a wide range of studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.