ArticlePloS one2011
The mechanism of enhanced insulin amyloid fibril formation by NaCl is better explained by a conformational change model.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 62 citations in OpenAlex.
- Investigation of the Effect of NaCl Concentrations on the Formation of Amyloid Fibrils During the Cooking of Wheat Noodles.Foods (Basel, Switzerland) · 2025Article
- Efficient encapsulation of insulin by a giant macrocycle as a powerful approach to the inhibition of its fibrillation.Chemical science · 2025Article
- Comparative study of the hydrophobic interaction effect of pH and ionic strength on aggregation/emulsification of Congo red and amyloid fibrillation of insulin.BBA advances · 2022Article
- Degradation of insulin amyloid by antibiotic minocycline and formation of toxic intermediates.Scientific reports · 2021Article
- Downstream processing of recombinant human insulin and its analogues production fromBioresources and bioprocessing · 2021Review
- Defects in Protein Folding and/or Quality Control Cause Protein Aggregation in the Endoplasmic Reticulum.Progress in molecular and subcellular biology · 2021Article
- Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods.Molecules (Basel, Switzerland) · 2020Review
- An ultrafast insulin formulation enabled by high-throughput screening of engineered polymeric excipients.Science translational medicine · 2020Article
- Pharmacokinetic Analysis of Epithelial/Endothelial Cell Barriers in Microfluidic Bilayer Devices with an Air-Liquid Interface.Micromachines · 2020Article
- Trehalose Effect on the Aggregation of Model Proteins into Amyloid Fibrils.Life (Basel, Switzerland) · 2020Article
- Anomalous Salt Dependence Reveals an Interplay of Attractive and Repulsive Electrostatic Interactions in α-synuclein Fibril Formation.QRB discovery · 2020Article
- A Small Molecule Impedes Insulin Fibrillation: Another New Role of Phenothiazine Derivatives.ChemistryOpen · 2018Article
- Inhibition of insulin fibrillation by osmolytes: Mechanistic insights.Scientific reports · 2015Article
- Accelerated insulin aggregation under alternating current electric fields: Relevance to amyloid kinetics.Biomicrofluidics · 2015Article
- Generating local amyloidosis in mice by the subcutaneous injection of human insulin amyloid fibrils.Experimental and therapeutic medicine · 2014Article
- Stepwise organization of the β-structure identifies key regions essential for the propagation and cytotoxicity of insulin amyloid fibrils.The Journal of biological chemistry · 2014Article
- Article
- Electrostatic effects in filamentous protein aggregation.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high propensity of insulin to fibrillate causes severe biomedical and biotechnological complications. Insulin fibrillation studies attain significant importance considering the prevalence of diabetes and the requirement of functional insulin in each dose. Although studied since the early years of the 20(th) century, elucidation of the mechanism of insulin fibrillation has not been understood completely. We have previously, through several studies, shown that insulin hexamer dissociates into monomer that undergoes partial unfolding before converting into mature fibrils. In this study we have established that NaCl enhances insulin fibrillation mainly due to subtle structural changes and is not a mere salt effect. We have carried out studies both in the presence and absence of urea and Gdn.HCl and compared the relationship between conformation of insulin induced by urea and Gdn.HCl with respect to NaCl at both pH 7.4 (hexamer) and pH 2 (monomer). Fibril formation was followed with a Thioflavin T assay and structural changes were monitored by circular dichroism and size-exclusion chromatography. The results show salt-insulin interactions are difficult to classify as commonly accepted Debye-Hückel or Hofmeister series interactions but instead a strong correlation between the association states and conformational states of insulin and their propensity to fibrillate is evident.
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