ArticlePloS one2011
Chromosome isolation by flow sorting in Aegilops umbellulata and Ae. comosa and their allotetraploid hybrids Ae. biuncialis and Ae. geniculata.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed.
- Dynamics of repetitive DNA sequences over the course of evolution and intraspecific divergence of tetraploid goat-grass species Aegilops biuncialis vis.BMC plant biology · 2025Article
- Perspectives on the utilization ofFrontiers in plant science · 2025Review
- DArTseq genotyping facilitates identification of Aegilops biuncialis chromatin introgressed into bread wheat Mv9kr1.Plant molecular biology · 2024Article
- Intraspecific divergence of diploid grassComparative cytogenetics · 2023Article
- Comparative genomic analysis of 5MFrontiers in plant science · 2023Article
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.BMC genomics · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- A robust KASP marker for selection of four pairs of linked leaf rust and stripe rust resistance genes introgressed on chromosome arm 5DS from different wheat genomes.Molecular biology reports · 2021Article
- Chromosome and Molecular Analyses Reveal Significant Karyotype Diversity and Provide New Evidence on the Origin ofPlants (Basel, Switzerland) · 2021Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Production and characterization of a disomic 1M/1DMolecular breeding : new strategies in plant improvement · 2021Article
- Aegilops umbellulata introgression carrying leaf rust and stripe rust resistance genes Lr76 and Yr70 located to 9.47-Mb region on 5DS telomeric end through a combination of chromosome sorting and sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Analysis of Structural Genomic Diversity inFrontiers in plant science · 2020Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- Characterization, identification and evaluation of a set of wheat-Aegilops comosa chromosome lines.Scientific reports · 2019Article
- The karyotype ofMolecular cytogenetics · 2019Article
- Development of intron targeting (IT) markers specific for chromosome arm 4VS of Haynaldia villosa by chromosome sorting and next-generation sequencing.BMC genomics · 2017Article
- Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship.PloS one · 2017Article
- Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization.Annals of botany · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted chromosomes, thus confirming the usefulness of this approach for physical mapping. The SSR markers are suitable for marker assisted selection of wheat-Aegilops introgression lines. The results obtained in this work provide new opportunities for dissecting genomes of wild relatives of wheat with the aim to assist in alien gene transfer and discovery of novel genes for wheat improvement.
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