ArticlePloS one2011
Identification and localization of Myxococcus xanthus porins and lipoproteins.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility.Science advances · 2023Article
- Bacterial Outer Membrane Polysaccharide Export (OPX) Proteins Occupy Three Structural Classes with Selective β-Barrel Porin Requirements for Polymer Secretion.Microbiology spectrum · 2022Article
- Myxobacterial Genomics and Post-Genomics: A Review of Genome Biology, Genome Sequences and Related 'Omics Studies.Microorganisms · 2021Review
- Kin recognition and outer membrane exchange (OME) in myxobacteria.Current opinion in microbiology · 2020Review
- MbnH is a diheme MauG-like protein associated with microbial copper homeostasis.The Journal of biological chemistry · 2019Article
- A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane.Nature communications · 2019Article
- Engineering Pseudochelin Production in Myxococcus xanthus.Applied and environmental microbiology · 2018Article
- Infectious polymorphic toxins delivered by outer membrane exchange discriminate kin in myxobacteria.eLife · 2017Article
- Myxobacteria: Moving, Killing, Feeding, and Surviving Together.Frontiers in microbiology · 2016Review
- Myxococcus CsgA, Drosophila Sniffer, and human HSD10 are cardiolipin phospholipases.Genes & development · 2015Article
- Contact- and Protein Transfer-Dependent Stimulation of Assembly of the Gliding Motility Machinery in Myxococcus xanthus.PLoS genetics · 2015Article
- Combinatorial regulation of the dev operon by MrpC2 and FruA during Myxococcus xanthus development.Journal of bacteriology · 2015Article
- A genetic screen in Myxococcus xanthus identifies mutants that uncouple outer membrane exchange from a downstream cellular response.Journal of bacteriology · 2014Article
- Synthesis and assembly of a novel glycan layer in Myxococcus xanthus spores.The Journal of biological chemistry · 2014Article
- Genetic redundancy, proximity, and functionality of lspA, the target of antibiotic TA, in the Myxococcus xanthus producer strain.Journal of bacteriology · 2014Article
- Phase variation in Myxococcus xanthus yields cells specialized for iron sequestration.PloS one · 2014Article
- The lethal cargo of Myxococcus xanthus outer membrane vesicles.Frontiers in microbiology · 2014Article
- Molecular recognition in myxobacterial outer membrane exchange: functional, social and evolutionary implications.Molecular microbiology · 2014Review
- Comparative genomics of transport proteins in developmental bacteria: Myxococcus xanthus and Streptomyces coelicolor.BMC microbiology · 2013Article
- Insights into xanthomonas axonopodis pv. citri biofilm through proteomics.BMC microbiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myxococcus xanthus DK1622 contains inner (IM) and outer membranes (OM) separated by a peptidoglycan layer. Integral membrane, β-barrel proteins are found exclusively in the OM where they form pores allowing the passage of nutrients, waste products and signals. One porin, Oar, is required for intercellular communication of the C-signal. An oar mutant produces CsgA but is unable to ripple or stimulate csgA mutants to develop suggesting that it is the channel for C-signaling. Six prediction programs were evaluated for their ability to identify β-barrel proteins. No program was reliable unless the predicted proteins were first parsed using Signal P, Lipo P and TMHMM, after which TMBETA-SVM and TMBETADISC-RBF identified β-barrel proteins most accurately. 228 β-barrel proteins were predicted from among 7331 protein coding regions, representing 3.1% of total genes. Sucrose density gradients were used to separate vegetative cell IM and OM fractions, and LC-MS/MS of OM proteins identified 54 β-barrel proteins. Another class of membrane proteins, the lipoproteins, are anchored in the membrane via a lipid moiety at the N-terminus. 44 OM proteins identified by LC-MS/MS were predicted lipoproteins. Lipoproteins are distributed between the IM, OM and ECM according to an N-terminal sorting sequence that varies among species. Sequence analysis revealed conservation of alanine at the +7 position of mature ECM lipoproteins, lysine at the +2 position of IM lipoproteins, and no noticable conservation within the OM lipoproteins. Site directed mutagenesis and immuno transmission electron microscopy showed that alanine at the +7 position is essential for sorting of the lipoprotein FibA into the ECM. FibA appears at normal levels in the ECM even when a +2 lysine is added to the signal sequence. These results suggest that ECM proteins have a unique method of secretion. It is now possible to target lipoproteins to specific IM, OM and ECM locations by manipulating the amino acid sequence near the +1 cysteine processing site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.