ArticlePloS one2011
Enhanced Hsp70 expression protects against acute lung injury by modulating apoptotic pathways.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Fenofibrate Mitigates Acute Lung Injury in a Rat Model of Feces-Induced Peritonitis.International journal of molecular sciences · 2026Article
- Mitochondrial-related biomarkers as the diagnostic markers in sepsis induced acute respiratory distress syndrome.European journal of medical research · 2026Article
- Unraveling the Impact ofLife (Basel, Switzerland) · 2024Article
- HSP70 Is a Critical Regulator of HSP90 Inhibitor's Effectiveness in Preventing HCl-Induced Chronic Lung Injury and Pulmonary Fibrosis.International journal of molecular sciences · 2024Article
- RBM3 is associated with acute lung injury in septic mice and patients via the NF-κB/NLRP3 pathway.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023Article
- The Ameliorative Effects of Ascorbic Acid on Critical Illness Polyneuropathy in Rodent Sepsis Model.Journal of pediatric intensive care · 2020Article
- Melatonin receptor agonist protects against acute lung injury induced by ventilator through up-regulation of IL-10 production.Respiratory research · 2020Article
- Moderate Fever Cycles as a Potential Mechanism to Protect the Respiratory System in COVID-19 Patients.Frontiers in medicine · 2020Review
- TLR3 absence confers increased survival with improved macrophage activity against pneumonia.JCI insight · 2019Article
- Article
- The 70-kDa heat shock protein (Hsp70) as a therapeutic target for stroke.Expert opinion on therapeutic targets · 2018Review
- Cold-inducible RNA-binding protein (CIRP) causes sepsis-associated acute lung injury via induction of endoplasmic reticulum stress.Scientific reports · 2017Article
- Host responses to concurrent combined injuries in non-human primates.Journal of inflammation (London, England) · 2017Article
- The Role of the Membrane-Initiated Heat Shock Response in Cancer.Frontiers in molecular biosciences · 2016Review
- The detection and role of heat shock protein 70 in various nondisease conditions and disease conditions: a literature review.Cell stress & chaperones · 2015Review
- Stimulation of Brain AMP-Activated Protein Kinase Attenuates Inflammation and Acute Lung Injury in Sepsis.Molecular medicine (Cambridge, Mass.) · 2015Article
- Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs.Cell stress & chaperones · 2015Article
- TAT-HSP70 Attenuates Experimental Lung Injury.Shock (Augusta, Ga.) · 2015Article
- Multi-layered molecular mechanisms of polypeptide holding, unfolding and disaggregation by HSP70/HSP110 chaperones.Frontiers in molecular biosciences · 2015Review
- Heat shock protein 72 expressing stress in sepsis: unbridgeable gap between animal and human studies--a hypothetical "comparative" study.BioMed research international · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Acute respiratory distress syndrome (ARDS) is a highly lethal inflammatory lung disorder. Apoptosis plays a key role in its pathogenesis. We showed that an adenovirus expressing the 70 kDa heat shock protein Hsp70 (AdHSP) protected against sepsis-induced lung injury. In this study we tested the hypothesis that AdHSP attenuates apoptosis in sepsis-induced lung injury. Sepsis was induced in rats via cecal ligation and double puncture (2CLP). At the time of 2CLP PBS, AdHSP or AdGFP (an adenoviral vector expressing green fluorescent protein) were injected into the tracheas of septic rats. 48 hours later, lungs were isolated. One lung was fixed for TUNEL staining and immunohistochemistry. The other was homogenized to isolate cytosolic and nuclear protein. Immunoblotting, gel filtration and co-immunoprecipitation were performed in these extracts. In separate experiments MLE-12 cells were incubated with medium, AdHSP or AdGFP. Cells were stimulated with TNFα. Cytosolic and nuclear proteins were isolated. These were subjected to immunoblotting, co-immunoprecipitation and a caspase-3 activity assay. TUNEL assay demonstrated that AdHSP reduced alveolar cell apoptosis. This was confirmed by immunohistochemical detection of caspase 3 abundance. In lung isolated from septic animals, immunoblotting, co-immunoprecipitation and gel filtration studies revealed an increase in cytoplasmic complexes containing caspases 3, 8 and 9. AdHSP disrupted these complexes. We propose that Hsp70 impairs apoptotic cellular pathways via interactions with caspases. Disruption of large complexes resulted in stabilization of lower molecular weight complexes, thereby, reducing nuclear caspase-3. Prevention of apoptosis in lung injury may preserve alveolar cells and aid in recovery.
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Registered trials
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