ArticlePloS one2011
Designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- PEPTERGENT: A Peptide-Based Reagent for Detergent-Free Extraction of Membrane Proteins and Purification of Membrane Proteomes.Bio-protocol · 2026Article
- Towards Enhanced Tunability of Aqueous Biphasic Systems: Furthering the Grasp of Fluorinated Ionic Liquids in the Purification of Proteins.International journal of molecular sciences · 2024Article
- Simultaneous Purification of Human Interferon Alpha-2b and Serum Albumin Using Bioprivileged Fluorinated Ionic Liquid-Based Aqueous Biphasic Systems.International journal of molecular sciences · 2024Article
- Disclosing the Potential of Fluorinated Ionic Liquids as Interferon-Alpha 2b Delivery Systems.Nanomaterials (Basel, Switzerland) · 2022Article
- The G protein coupled receptor CXCR4 designed by the QTY code becomes more hydrophilic and retains cell signaling activity.Scientific reports · 2020Article
- Self-assembling peptides: From a discovery in a yeast protein to diverse uses and beyond.Protein science : a publication of the Protein Society · 2020Article
- Review
- Cell-Free Expression and Photo-Crosslinking of the Human Neuropeptide YFrontiers in pharmacology · 2019Article
- Insight into the Self-Assembling Properties of Peptergents: A Molecular Dynamics Simulation Study.International journal of molecular sciences · 2018Article
- The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution.eLife · 2018Article
- Peptide self-assembly into lamellar phases and the formation of lipid-peptide nanostructures.Nano research · 2018Article
- Amphiphilic peptides as novel nanomaterials: design, self-assembly and application.International journal of nanomedicine · 2018Review
- Discovery and design of self-assembling peptides.Interface focus · 2017Review
- Lipid-like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies.Chembiochem : a European journal of chemical biology · 2017Article
- Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Cell-free expression, purification, and ligand-binding analysis of Drosophila melanogaster olfactory receptors DmOR67a, DmOR85b and DmORCO.Scientific reports · 2015Article
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- Optimization of the recombinant production and purification of a self-assembling peptide in Escherichia coli.Microbial cell factories · 2014Article
- High-level soluble expression of one model olfactory receptor (ODR-10) in Escherichia coli cell-free system.World journal of microbiology & biotechnology · 2014Article
- Anthrax toxin-induced rupture of artificial lipid bilayer membranes.The Journal of chemical physics · 2013Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.