ArticleFrontiers in pharmacology2011
State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening.
Article in Frontiers in pharmacology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- A modular method for high-throughput measurement of ion channel currents in cardiac myocytes.Nature protocols · 2026Review
- hERGAT: predicting hERG blockers using graph attention mechanism through atom- and molecule-level interaction analyses.Journal of cheminformatics · 2025Article
- High-throughput methods for cardiac cellular electrophysiology studies: the road to personalized medicine.American journal of physiology. Heart and circulatory physiology · 2024Review
- Toward Quantitative Models in Safety Assessment: A Case Study to Show Impact of Dose-Response Inference on hERG Inhibition Models.International journal of molecular sciences · 2022Article
- Article
- Development of ASIC1a ligand-gated ion channel drug screening assays across multiple automated patch clamp platforms.Frontiers in molecular neuroscience · 2022Article
- There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments.Frontiers in molecular neuroscience · 2022Article
- Advancing Ion Channel Research with Automated Patch Clamp (APC) Electrophysiology Platforms.Advances in experimental medicine and biology · 2021Article
- 3D Printed Lab-on-a-Chip Platform for Chemical Stimulation and Parallel Analysis of Ion Channel Function.Micromachines · 2019Article
- Capsule Networks Showed Excellent Performance in the Classification of hERG Blockers/Nonblockers.Frontiers in pharmacology · 2019Article
- Using automated patch clamp electrophysiology platforms in pain-related ion channel research: insights from industry and academia.British journal of pharmacology · 2018Review
- Cardiomyocytes from human pluripotent stem cells: From laboratory curiosity to industrial biomedical platform.Biochimica et biophysica acta · 2016Article
- Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC³s).Micromachines · 2016Review
- Automated Electrophysiological and Pharmacological Evaluation of Human Pluripotent Stem Cell-Derived Cardiomyocytes.Stem cells and development · 2016Article
- Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp.BMC pharmacology & toxicology · 2015Article
- Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs.Scientific reports · 2015Article
- Novel screening techniques for ion channel targeting drugs.Channels (Austin, Tex.) · 2015Review
- Pluripotent stem cells as a platform for cardiac arrhythmia drug screening.Current treatment options in cardiovascular medicine · 2014Article
- Early identification of hERG liability in drug discovery programs by automated patch clamp.Frontiers in pharmacology · 2014Article
- Prototype for automatable, dielectrophoretically-accessed intracellular membrane-potential measurements by metal electrodes.Assay and drug development technologies · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.