ArticleMethods in molecular biology (Clifton, N.J.)2012
Detection of calcium sparks in intact and permeabilized skeletal muscle fibers.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Improper Remodeling of Organelles Deputed to CaInternational journal of molecular sciences · 2021Review
- Integrating Bioelectrical Currents and CaBioelectricity · 2020Review
- The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle.JCI insight · 2018Article
- Article
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Assessment of calcium sparks in intact skeletal muscle fibers.Journal of visualized experiments : JoVE · 2014Article
- Epidermal keratinocyte polarity and motility require Ca²⁺ influx through TRPV1.Journal of cell science · 2013Article
- Expression levels of sarcolemmal membrane repair proteins following prolonged exercise training in mice.Indian journal of biochemistry & biophysics · 2013Article
- Analysis of osmotic stress induced Ca2+ spark termination in mammalian skeletal muscle.Indian journal of biochemistry & biophysics · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Ca(2+) sparks are the elementary units of Ca(2+) signaling in striated muscle fibers that appear as highly localized Ca(2+) release events through ryanodine receptor (RyR) Ca(2+) release channels in the sarcoplasmic reticulum (SR). While these events are commonly observed in resting cardiac myocytes, they are rarely seen in resting skeletal muscle fibers. Since Ca(2+) spark analysis can provide extensive data on the Ca(2+) handling characteritsics of normal and diseased striated muscle, there has been interest in developing methods for observing Ca(2+) sparks in skeletal muscle. Previously, we discovered that stress generated by osmotic pressure changes induces a robust Ca(2+) spark response confined in close spatial proximity to the sarcolemmal membrane in wild-type intact mammalian muscles. Our studies showed these peripheral Ca(2+) sparks (PCS) were altered in dystrophic or aged skeletal muscles. Other methods to induce Ca(2+) sparks include permeabilization of the sarcolemmal membrane with detergents, such as saponin. In this chapter, we will discuss the methods for isolation of muscle fibers, the techniques for inducing Ca(2+) sparks in these isolated fibers, and provide guidance on the analysis of data from these experiments.
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