ArticleMethods in molecular biology (Clifton, N.J.)2012
Isolation and characterization of human fetal myoblasts.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.Frontiers in cell and developmental biology · 2021Article
- Truncating Variant inFrontiers in genetics · 2019Article
- Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS).Methods in molecular biology (Clifton, N.J.) · 2019Article
- Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations.Stem cells international · 2019Article
- Reporter-Based Isolation of Developmental Myogenic Progenitors.Frontiers in physiology · 2018Article
- Isolation of Primary Human Skeletal Muscle Cells.Bio-protocol · 2017Article
- Repression of phosphatidylinositol transfer protein α ameliorates the pathology of Duchenne muscular dystrophy.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies.Cell stem cell · 2016Article
- The multifaceted role of CD146/MCAM in the promotion of melanoma progression.Cancer cell international · 2015Article
- POMK mutations disrupt muscle development leading to a spectrum of neuromuscular presentations.Human molecular genetics · 2014Article
- Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Mouse regenerating myofibers detected as false-positive donor myofibers with anti-human spectrin.Human gene therapy · 2014Article
- Substrate stiffness affects skeletal myoblast differentiationScience and technology of advanced materials · 2012Article
- Human fetal skeletal muscle contains a myogenic side population that expresses the melanoma cell-adhesion molecule.Human molecular genetics · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Dissociated human fetal skeletal muscle contains myogenic cells, as well as non-myogenic cells such as adipocytes, fibroblasts, and lymphocytes. It is therefore important to determine an efficient and reliable isolation method to obtain a purer population of myoblasts. Toward this end, fluorescence-activated cell sorting in conjunction with robust myogenic cell surface markers can be utilized to enrich for myoblasts in dissociated muscle. In this chapter, we describe a method to significantly enrich for myoblasts using -melanoma cell adhesion molecule (MCAM), which we have determined to be an excellent marker of human fetal myoblasts. The myoblasts resulting from this isolation method can then be expanded in vitro and still retain significant myogenic activity as shown by an in vitro fusion assay. The ability to isolate a highly myogenic population from dissociated muscle facilitates the in vitro study of skeletal muscle development and muscle diseases. Furthermore, robust expansion of these cells will lead to new insights in the development of cell-based therapies for human muscle disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.