ArticleThe Journal of biological chemistry2012
Identification of potential tumor differentiation factor (TDF) receptor from steroid-responsive and steroid-resistant breast cancer cells.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- The Clinicopathological Significance of BiP/GRP-78 in Breast Cancer: A Meta-Analysis of Public Datasets and Immunohistochemical Detection.Current oncology (Toronto, Ont.) · 2022Pooled it
- Mass Spectrometry- and Structural Biology-Based Investigation of Proteins.Advances in experimental medicine and biology · 2026Review
- Mass Spectrometry for Proteomics-Based Investigation.Advances in experimental medicine and biology · 2026Review
- House dust mite sensitization drives cross-reactive immune responses to homologous helminth proteins.PLoS pathogens · 2021Article
- Identification of a novel TPM4 isoform transcript and comparison to the expression of other tropomyosin isoforms in bovine cardiac and skeletal muscles.International journal of biochemistry and molecular biology · 2021Article
- Targeting the GRP78 Pathway for Cancer Therapy.Frontiers in medicine · 2020Review
- Lactoferrin translocates to the nucleus of bovine rectal epithelial cells in the presence of Escherichia coli O157:H7.Veterinary research · 2019Article
- A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion.Scientific reports · 2018Article
- Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin.Molecular biology of the cell · 2018Article
- Ubiquitin Receptor RPN13 Mediates the Inhibitory Interaction of Diphenyldihaloketones CLEFMA and EF24 With the 26S Proteasome.Frontiers in chemistry · 2018Article
- Atrial electrophysiological and molecular remodelling induced by obstructive sleep apnoea.Journal of cellular and molecular medicine · 2017Article
- Mass spectrometry-based proteomics of oxidative stress: Identification of 4-hydroxy-2-nonenal (HNE) adducts of amino acids using lysozyme and bovine serum albumin as model proteins.Electrophoresis · 2016Article
- Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4Molecular biology international · 2016Article
- GRP78 is a novel receptor initiating a vascular barrier protective response to oxidized phospholipids.Molecular biology of the cell · 2014Article
- Protein-protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches.Cellular and molecular life sciences : CMLS · 2014Review
- Structural evaluation and analyses of tumor differentiation factor.The protein journal · 2013Article
- Characterization of tumor differentiation factor (TDF) and its receptor (TDF-R).Cellular and molecular life sciences : CMLS · 2013Review
- Investigation of stable and transient protein-protein interactions: Past, present, and future.Proteomics · 2013Review
- Mass spectrometry for the detection of potential psychiatric biomarkers.Journal of molecular psychiatry · 2013Review
- Mass spectrometry as a tool for studying autism spectrum disorder.Journal of molecular psychiatry · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor differentiation factor (TDF) is a recently discovered protein, produced by the pituitary gland and secreted into the bloodstream. TDF and TDF-P1, a 20-amino acid peptide selected from the open reading frame of TDF, induce differentiation in human breast and prostate cancer cells but not in other cells. TDF protein has no identified site of action or receptor, and its mechanism of action is unknown. Here, we used TDF-P1 to purify and identify potential TDF receptor (TDF-R) candidates from MCF7 steroid-responsive breast cancer cells and non-breast HeLa cancerous cells using affinity purification chromatography (AP), and mass spectrometry (MS). We identified four candidate proteins from the 70-kDa heat shock protein (HSP70) family in MCF7 cells. Experiments in non-breast HeLa cancerous cells did not identify any TDF-R candidates. AP and MS experiments were validated by AP and Western blotting (WB). We additionally looked for TDF-R in steroid-resistant BT-549 cells and human dermal fibroblasts (HDF-a) using AP and WB. TDF-P1 interacts with potential TDF-R candidates from MCF7 and BT-549 breast cells but not from HeLa or HDF-a cells. Immunofluorescence (IF) experiments identified GRP78, a TDF-R candidate, at the cell surface of MCF7, BT-549 breast cells, and HeLa cells but not HDF-a cells. IF of other HSP70 proteins demonstrated labeling on all four cell types. These results point toward GRP78 and HSP70 proteins as strong TDF-R candidates and suggest that TDF interacts with its receptor, exclusively on breast cells, through a steroid-independent pathway.
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Registered trials
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