ArticleThe Journal of biological chemistry2012
Molecular cloning and functional characterization of Xenopus tropicalis frog transient receptor potential vanilloid 1 reveal its functional evolution for heat, acid, and capsaicin sensitivities in terrestrial vertebrates.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 48 citations in OpenAlex.
- Physiological functions and pharmacological targeting of transient receptor potential channels.Pharmacological reviews · 2025Review
- Article
- Functional analysis of thermo-sensitive TRPV1 in an aquatic vertebrate, masu salmon (Biochemistry and biophysics reports · 2022Article
- A single TRPV1 amino acid controls species sensitivity to capsaicin.Scientific reports · 2020Article
- The Mysteries of Capsaicin-Sensitive Afferents.Frontiers in physiology · 2020Review
- Capsaicin Is a Negative Allosteric Modulator of the 5-HTFrontiers in pharmacology · 2020Article
- Two Vanilloid Ligand Bindings Per Channel Are Required to Transduce Capsaicin-Activating Stimuli.Frontiers in molecular neuroscience · 2019Article
- TRPs et al.: a molecular toolkit for thermosensory adaptations.Pflugers Archiv : European journal of physiology · 2018Review
- Article
- Evolutionary tuning of TRPA1 and TRPV1 thermal and chemical sensitivity in vertebrates.Temperature (Austin, Tex.) · 2017Review
- Evolution of Heat Sensors Drove Shifts in Thermosensation between Xenopus Species Adapted to Different Thermal Niches.The Journal of biological chemistry · 2016Article
- The Xenopus tropicalis orthologue of TRPV3 is heat sensitive.The Journal of general physiology · 2015Article
- Evolutionary dynamics of metazoan TRP channels.Pflugers Archiv : European journal of physiology · 2015Review
- Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons.Molecular pain · 2015Article
- Molecular basis determining inhibition/activation of nociceptive receptor TRPA1 protein: a single amino acid dictates species-specific actions of the most potent mammalian TRPA1 antagonist.The Journal of biological chemistry · 2014Article
- Identification of molecular determinants for a potent mammalian TRPA1 antagonist by utilizing species differences.Journal of molecular neuroscience : MN · 2013Article
- Effects of sciatic nerve transection on ultrastructure, NADPH-diaphorase reaction and serotonin-, tyrosine hydroxylase-, c-Fos-, glucose transporter 1- and 3-like immunoreactivities in frog dorsal root ganglion.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2013Article
- The zebrafish ortholog of TRPV1 is required for heat-induced locomotion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Analysis of transient receptor potential ankyrin 1 (TRPA1) in frogs and lizards illuminates both nociceptive heat and chemical sensitivities and coexpression with TRP vanilloid 1 (TRPV1) in ancestral vertebrates.The Journal of biological chemistry · 2012Article
- TRP channels: a missing bond in the entrainment mechanism of peripheral clocks throughout evolution.Temperature (Austin, Tex.)Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional difference of thermosensitive transient receptor potential (TRP) channels in the evolutionary context has attracted attention, but thus far little information is available on the TRP vanilloid 1 (TRPV1) function of amphibians, which diverged earliest from terrestrial vertebrate lineages. In this study we cloned Xenopus tropicalis frog TRPV1 (xtTRPV1), and functional characterization was performed using HeLa cells heterologously expressing xtTRPV1 (xtTRPV1-HeLa) and dorsal root ganglion neurons isolated from X. tropicalis (xtDRG neurons) by measuring changes in the intracellular calcium concentration ([Ca(2+)](i)). The channel activity was also observed in xtTRPV1-expressing Xenopus oocytes. Furthermore, we tested capsaicin- and heat-induced nocifensive behaviors of the frog X. tropicalis in vivo. At the amino acid level, xtTRPV1 displays ∼60% sequence identity to other terrestrial vertebrate TRPV1 orthologues. Capsaicin induced [Ca(2+)](i) increases in xtTRPV1-HeLa and xtDRG neurons and evoked nocifensive behavior in X. tropicalis. However, its sensitivity was extremely low compared with mammalian orthologues. Low extracellular pH and heat activated xtTRPV1-HeLa and xtDRG neurons. Heat also evoked nocifensive behavior. In oocytes expressing xtTRPV1, inward currents were elicited by heat and low extracellular pH. Mutagenesis analysis revealed that two amino acids (tyrosine 523 and alanine 561) were responsible for the low sensitivity to capsaicin. Taken together, our results indicate that xtTRPV1 functions as a polymodal receptor similar to its mammalian orthologues. The present study demonstrates that TRPV1 functions as a heat- and acid-sensitive channel in the ancestor of terrestrial vertebrates. Because it is possible to examine vanilloid and heat sensitivities in vitro and in vivo, X. tropicalis could be the ideal experimental lower vertebrate animal for the study of TRPV1 function.
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