ArticleJournal of virology2012
Accelerated shedding of prions following damage to the olfactory epithelium.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Detection of Pathognomonic Biomarker PrPBiomolecules · 2020Review
- Very low oral exposure to prions of brain or saliva origin can transmit chronic wasting disease.PloS one · 2020Article
- The cellular prion protein promotes olfactory sensory neuron survival and axon targeting during adult neurogenesis.Developmental biology · 2018Article
- The olfactory bulb as the entry site for prion-like propagation in neurodegenerative diseases.Neurobiology of disease · 2018Article
- How do PrPPathogens (Basel, Switzerland) · 2017Review
- The Pathogenic A116V Mutation Enhances Ion-Selective Channel Formation by Prion Protein in Membranes.Biophysical journal · 2016Article
- Seeded Amplification of Chronic Wasting Disease Prions in Nasal Brushings and Recto-anal Mucosa-Associated Lymphoid Tissues from Elk by Real-Time Quaking-Induced Conversion.Journal of clinical microbiology · 2016Article
- Antemortem Detection of Chronic Wasting Disease Prions in Nasal Brush Collections and Rectal Biopsy Specimens from White-Tailed Deer by Real-Time Quaking-Induced Conversion.Journal of clinical microbiology · 2016Article
- Detection of Atypical H-Type Bovine Spongiform Encephalopathy and Discrimination of Bovine Prion Strains by Real-Time Quaking-Induced Conversion.Journal of clinical microbiology · 2016Article
- Host Determinants of Prion Strain Diversity Independent of Prion Protein Genotype.Journal of virology · 2015Article
- Lesion of the olfactory epithelium accelerates prion neuroinvasion and disease onset when prion replication is restricted to neurons.PloS one · 2015Article
- A test for Creutzfeldt-Jakob disease using nasal brushings.The New England journal of medicine · 2014Article
- Axonal and transynaptic spread of prions.Journal of virology · 2014Article
- Mucosal transmission and pathogenesis of chronic wasting disease in ferrets.The Journal of general virology · 2013Article
- Prion disease and the innate immune system.Viruses · 2012Review
- Article
- Article
- Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
In this study, we investigated the role of damage to the nasal mucosa in the shedding of prions into nasal samples as a pathway for prion transmission. Here, we demonstrate that prions can replicate to high levels in the olfactory sensory epithelium (OSE) in hamsters and that induction of apoptosis in olfactory receptor neurons (ORNs) in the OSE resulted in sloughing off of the OSE from nasal turbinates into the lumen of the nasal airway. In the absence of nasotoxic treatment, olfactory marker protein (OMP), which is specific for ORNs, was not detected in nasal lavage samples. However, after nasotoxic treatment that leads to apoptosis of ORNs, both OMP and prion proteins were present in nasal lavage samples. The cellular debris that was released from the OSE into the lumen of the nasal airway was positive for both OMP and the disease-specific isoform of the prion protein, PrP(Sc). By using the real-time quaking-induced conversion assay to quantify prions, a 100- to 1,000-fold increase in prion seeding activity was observed in nasal lavage samples following nasotoxic treatment. Since neurons replicate prions to higher levels than other cell types and ORNs are the most environmentally exposed neurons, we propose that an increase in ORN apoptosis or damage to the nasal mucosa in a host with a preexisting prion infection of the OSE could lead to a substantial increase in the release of prion infectivity into nasal samples. This mechanism of prion shedding from the olfactory mucosa could contribute to prion transmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.