ArticleAging cell2012
Characterization of cellular senescence mechanisms in human corneal endothelial cells.
Article in Aging cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Bioengineering Strategies for Corneal Endothelial Cell Injection Therapy: Advances, Challenges, and Clinical Translation.Bioengineering (Basel, Switzerland) · 2025Review
- Spatiotemporal single-cell analysis elucidates the cellular and molecular dynamics of human cornea aging.Genome medicine · 2025Article
- Review
- Transcriptomic comparison of corneal endothelial cells in young versus old corneas.Scientific reports · 2024Article
- Bioinformatics analysis of signature genes related to cell death in keratoconus.Scientific reports · 2024Article
- Role of NADPH Oxidase 4 in Corneal Endothelial Cells Is Mediated by Endoplasmic Reticulum Stress and Autophagy.Antioxidants (Basel, Switzerland) · 2023Article
- A p-Tyr42 RhoA Inhibitor Promotes the Regeneration of Human Corneal Endothelial Cells by Ameliorating Cellular Senescence.Antioxidants (Basel, Switzerland) · 2023Article
- Harnessing the power of Raman spectroscopic imaging for ophthalmology.Frontiers in chemistry · 2023Review
- Proliferation Increasing Genetic Engineering in Human Corneal Endothelial Cells: A Literature Review.Frontiers in medicine · 2021Review
- Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion.Scientific reports · 2020Article
- Oligonucleotides targeting TCF4 triplet repeat expansion inhibit RNA foci and mis-splicing in Fuchs' dystrophy.Human molecular genetics · 2018Article
- Ribonuclease 5 facilitates corneal endothelial wound healing via activation of PI3-kinase/Akt pathway.Scientific reports · 2016Article
- Oxidative stress and premature senescence in corneal endothelium following penetrating keratoplasty in an animal model.BMC ophthalmology · 2016Article
- Microarray analysis of cell cycle gene expression in adult human corneal endothelial cells.PloS one · 2014Article
- Tissue microarray analysis of cyclin-dependent kinase inhibitors p21 and p16 in Fuchs dystrophy.Cornea · 2013Article
- MicroRNA profile comparison of the corneal endothelia of young and old mice: implications for senescence of the corneal endothelium.Molecular vision · 2013Article
- Corneal endothelial cells provide evidence of accelerated cellular senescence associated with HIV infection: a case-control study.PloS one · 2013Article
- Telomerase immortalization of human corneal endothelial cells yields functional hexagonal monolayers.PloS one · 2012Article
- Research progress on the negative factors of corneal endothelial cells proliferation.International journal of ophthalmology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The human cornea is a tri-laminar structure composed of several cell types with substantial mitotic potential. Age-related changes in the cornea are associated with declining visual acuity and the onset of overt age-related corneal diseases. Corneal transplantation is commonly used to restore vision in patients with damaged or diseased corneas. However, the supply of donor tissue is limited, and thus there is considerable interest in the development of tissue-engineered alternatives. A major obstacle to these approaches is the short replicative lifespan of primary human corneal endothelial cells (HCEC). Accordingly, a comprehensive investigation of the signalling pathways and mechanisms underpinning proliferative lifespan and senescence in HCEC was undertaken. The effects of exogenous human telomerase reverse transcriptase expression, p53 knockdown, disruption of the pRb pathway by over-expression of CDK4 and reduced oxygen concentration on the lifespan of primary HCEC were evaluated. We provide proof-of-principle that forced expression of telomerase, when combined with either p53 knockdown or CDK4 over-expression, is sufficient to produce immortalized HCEC lines. The resultant cell lines express an HCEC-specific transcriptional fingerprint, and retain expression of the corneal endothelial temperature-sensitive potassium channel, suggesting that significant dedifferentiation does not occur as a result of these modes of immortalization. Exploiting these insights into proliferative lifespan barriers in HCEC will underpin the development of novel strategies for cell-based therapies in the human cornea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.