ArticleInternational journal of nanomedicine2011
Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process.
Article in International journal of nanomedicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
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- Small Cellular Particles from European Spruce Needle Homogenate.International journal of molecular sciences · 2023Article
- Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review.International journal of molecular sciences · 2023Review
- Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy.International journal of molecular sciences · 2022Article
- Removal of platelets from blood plasma to improve the quality of extracellular vesicle research.Journal of thrombosis and haemostasis : JTH · 2022Article
- Application of Dynamic and Static Light Scattering for Size and Shape Characterization of Small Extracellular Nanoparticles in Plasma and Ascites of Ovarian Cancer Patients.International journal of molecular sciences · 2021Article
- Stability of Erythrocyte-Derived Nanovesicles Assessed by Light Scattering and Electron Microscopy.International journal of molecular sciences · 2021Article
- Autologous Platelet- and Extracellular Vesicle-Rich Plasma Is an Effective Treatment Modality for Chronic Postoperative Temporal Bone Cavity Inflammation: Randomized Controlled Clinical Trial.Frontiers in bioengineering and biotechnology · 2021Article
- Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon.International journal of nanomedicine · 2021Article
- Extracellular Vesicle Isolation Yields Increased by Low-Temperature Gaseous Plasma Treatment of Polypropylene Tubes.Polymers · 2020Article
- Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering.Cells · 2019Article
- Comparison of the size of membrane microparticles of different cellular origin by dynamic light scattering.Doklady. Biochemistry and biophysics · 2016Article
- Digital sensing and sizing of vesicular stomatitis virus pseudotypes in complex media: a model for Ebola and Marburg detection.ACS nano · 2014Article
- Red blood cell vesiculation in hereditary hemolytic anemia.Frontiers in physiology · 2013Review
- The role of extracellular vesicles in phenotypic cancer transformation.Radiology and oncology · 2013Review
- Interaction between dipolar lipid headgroups and charged nanoparticles mediated by water dipoles and ions.International journal of molecular sciences · 2013Article
- Monovalent ions and water dipoles in contact with dipolar zwitterionic lipid headgroups-theory and MD simulations.International journal of molecular sciences · 2013Article
- Standardization of sample collection, isolation and analysis methods in extracellular vesicle research.Journal of extracellular vesicles · 2013Article
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Authors and funding
16 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundShedding of nanoparticles from the cell membrane is a common process in all cells. These nanoparticles are present in body fluids and can be harvested by isolation. To collect circulating nanoparticles from blood, a standard procedure consisting of repeated centrifugation and washing is applied to the blood samples. Nanoparticles can also be shed from blood cells during the isolation process, so it is unclear whether nanoparticles found in the isolated material are present in blood at sampling or if are they created from the blood cells during the isolation process. We addressed this question by determination of the morphology and identity of nanoparticles harvested from blood.
methodsThe isolates were visualized by scanning electron microscopy, analyzed by flow cytometry, and nanoparticle shapes were determined theoretically.
resultsThe average size of nanoparticles was about 300 nm, and numerous residual blood cells were found in the isolates. The shapes of nanoparticles corresponded to the theoretical shapes obtained by minimization of the membrane free energy, indicating that these nanoparticles can be identified as vesicles. The concentration and size of nanoparticles in blood isolates was sensitive to the temperature during isolation. We demonstrated that at lower temperatures, the nanoparticle concentration was higher, while the nanoparticles were on average smaller.
conclusionThese results indicate that a large pool of nanoparticles is produced after blood sampling. The shapes of deformed blood cells found in the isolates indicate how fragmentation of blood cells may take place. The results show that the contents of isolates reflect the properties of blood cells and their interaction with the surrounding solution (rather than representing only nanoparticles present in blood at sampling) which differ in different diseases and may therefore present a relevant clinical parameter.
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