ArticleMolecular vision2011
Changes of the ocular surface and aquaporins in the lacrimal glands of rabbits during pregnancy.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Role of tear vasoactive intestinal peptide on dry eyes after laser keratorefractive surgery.BMC ophthalmology · 2023Trial
- Lute-GenAntioxidants (Basel, Switzerland) · 2026Article
- Aquaporin-4 Dysfunction in Depression: From Pathogenic Mechanisms to Novel Therapeutic Targeting.International journal of molecular sciences · 2026Review
- The Role of Aquaporin 4 in Lacrimal Gland Ductal Fluid Secretion in Mice.Investigative ophthalmology & visual science · 2024Article
- Evaluation of dry eye and meibomian gland function in pregnancy.International ophthalmology · 2023Article
- A case-control study of the ocular surface and tear film parameters in gestational diabetes mellitus.Indian journal of ophthalmology · 2023Article
- A Narrative Review of the Complex Relationship between Pregnancy and Eye Changes.Diagnostics (Basel, Switzerland) · 2021Review
- Dry eye, its clinical subtypes and associated factors in healthy pregnancy: A cross-sectional study.PloS one · 2021Article
- Effects of Topical Gabapentin on Ocular Pain and Tear Secretion.Frontiers in pharmacology · 2021Article
- Intraocular pressure and tear production changes in pregnant women at term pregnancy and immediate post-partum: A pilot study.Journal of family medicine and primary care · 2020Article
- Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery.PloS one · 2020Article
- The regulatory role of vasoactive intestinal peptide in lacrimal gland ductal fluid secretion: A new piece of the puzzle in tear production.Molecular vision · 2020Article
- An In Vitro Model for the Ocular Surface and Tear Film System.Scientific reports · 2017Article
- The Sustained Effects on Tear Volume of Pilocarpine Hydrochloride in Gelatin by Hydrogel Administered by An Implant-mediated Drug Delivery System.Current drug delivery · 2017Article
- ENaC in the Rabbit Lacrimal Gland and its Changes During Sjögren Syndrome and Pregnancy.Eye & contact lens · 2015Article
- Aquaporins in the eye: expression, function, and roles in ocular disease.Biochimica et biophysica acta · 2014Review
- Diverse mediators modulate the chloride ion fluxes that drive lacrimal fluid production.Investigative ophthalmology & visual science · 2013Article
- Na(+)/K(+)-ATPase expression changes in the rabbit lacrimal glands during pregnancy.Current eye research · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo test the hypotheses that pregnancy represents a physiologic condition that is associated with dry eye symptoms, and the expression of aquaporin 4 (AQP4) and AQP5 are altered in the lacrimal gland (LG) from term pregnant rabbits.
methodsSchirmer's test, tear break-up time (BUT), and Rose Bengal staining were used to evaluate ocular surface health. LG were obtained from term pregnant rabbits and age-matched female control rabbits and then processed for laser capture microdissection (LCM), real time RT-PCR, western blot, and immunofluorescence for the detection and quantification of mRNA and proteins of AQP4 and AQP5.
resultsPregnant rabbits demonstrated typical clinical symptoms of dry eye, including decreased Schirmer score and BUT as well as increased Rose Bengal staining of cornea. In term pregnant rabbits, mRNA for AQP5 from whole LG was significantly lower than that of control rabbits, while mRNA for AQP4 was not. Levels of mRNA for AQP4 and AQP5 underwent significant changes in acini and epithelial cells from specific duct segments during pregnancy. Western blot from whole LG lysates demonstrated that expression of AQP4 was 24% more abundant in term pregnant rabbits while AQP5 was 22% less when compared to control rabbits respectively. At term pregnancy, AQP4 immunoreactivity (AQP4-IR) was increased in acini while its intensity remained the same in ducts. AQP5-IR was present in both apical and basolateral membranes of acinar cells in normal control and pregnant rabbits, while ductal cells in pregnant rabbits also showed significant amount of AQP5-IR.
conclusionsThe data presented here demonstrated significant dry eye symptoms in pregnant rabbits. Our data also showed altered expressions of AQP4 and AQP5 during pregnancy and suggested that these changes may contribute to the altered LG secretion and dry eye symptoms during pregnancy.
Indexed as
Identifiers
22128232PMC3224838W2120489631What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.