ArticleThe Journal of biological chemistry2012
Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
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- Metal Messengers: Communication in the Bacterial World through Transition-Metal-Sensing Two-Component Systems.Biochemistry · 2023Review
- Genetic and environmental determinants of surface adaptations inMicrobiology (Reading, England) · 2023Article
- The Alginate and Motility Regulator AmrZ is Essential for the Regulation of the Dispersion Response bymSphere · 2022Article
- Controlling Biofilm Development Through Cyclic di-GMP Signaling.Advances in experimental medicine and biology · 2022Review
- Conquering the host:Frontiers in microbiology · 2022Review
- SagS and its unorthodox contributions toBiofilm · 2021Review
- Two-Component Signaling Systems Regulate Diverse Virulence-Associated Traits in Pseudomonas aeruginosa.Applied and environmental microbiology · 2021Article
- Colony spreading of the gliding bacterium Flavobacterium johnsoniae in the absence of the motility adhesin SprB.Scientific reports · 2021Article
- Recent evidence for multifactorial biofilm regulation by heme sensor proteins NosP and H-NOX.Chemistry letters · 2021Article
- STAS Domain Only Proteins in Bacterial Gene Regulation.Frontiers in cellular and infection microbiology · 2021Review
- Connected partner-switches control the life style of Pseudomonas aeruginosa through RpoS regulation.Scientific reports · 2019Article
- Two Component Regulatory Systems and Antibiotic Resistance in Gram-Negative Pathogens.International journal of molecular sciences · 2019Review
- Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming.iScience · 2019Article
- Two-Component Signal Transduction Systems: A Major Strategy for Connecting Input Stimuli to Biofilm Formation.Frontiers in microbiology · 2018Review
- Protection of the general stress response σThe Journal of biological chemistry · 2017Article
- Two-component systems required for virulence in Pseudomonas aeruginosa.FEMS microbiology letters · 2017Review
- The General Stress Response σS Is Regulated by a Partner Switch in the Gram-negative Bacterium Shewanella oneidensis.The Journal of biological chemistry · 2016Article
- The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The histidine-containing phosphotransfer protein-B (HptB; PA3345) is an intermediate protein involved in transferring a phosphoryl group from multiple sensor kinases to the response regulator PA3346 in Pseudomonas aeruginosa PAO1. The objective of this study was to elucidate the biological significance of the HptB-PA3346 interaction and the regulatory mechanisms thereafter. The transcription profiling analysis of an hptB knock-out mutant showed that the expression of a number of motility-related genes was altered consistent with the non-swarming phenotype observed for the mutant. Domain analysis indicated that the PA3346 C-terminal region (PA3346C) exhibits ∼30% identity with the anti-σ factor SpoIIAB of Bacillus subtilis. The presence of Ser/Thr protein kinase activity targeting an anti-σ antagonist, PA3347, at Ser-56 was confirmed in PA3346C using an in vitro phosphorelay assay. Furthermore, PA3346C and the anti-σ(28) factor FlgM were found to interact with PA3347 individually both in vivo and in vitro. FlgM displaced PA3346C in binding of PA3347 and was then competitively displaced by σ(28) from the PA3347-FlgM complex, forming a phosphorylation-dependent partner-switching system. The significance of PA3347 phosphorylation in linking the partner-switching system and swarming motility was established by analyzing the swarming phenotype of the PA3347 knock-out mutant and its complement strains.
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