ArticleNucleic acids research2012
Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 81 citations in OpenAlex.
- Hybridization-Based Electrochemical Biosensors for RNA Detection: Key Concepts and Insights.Sensors and actuators. B, Chemical · 2026Article
- A dexterous fluorescence sensor for diversified-biomarker detection using cyclic EDC-CHA (EDCHA) system.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- A sensitive one-pot ROA assay for rapid miRNA detection.aBIOTECH · 2024Article
- Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3).Microbial cell factories · 2023Article
- Revealing an initiation inhibition of RCA and its application in nucleic acid detection.Acta biochimica et biophysica Sinica · 2023Article
- Programmable mismatch-fueled high-efficiency DNA signal amplifier.Chemical science · 2022Article
- Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variants.Biosensors & bioelectronics · 2022Article
- Rapid quantification of microRNA-375 through one-pot primer-generating rolling circle amplification.The Analyst · 2022Article
- Detection of SARS-CoV-2 RNA through tandem isothermal gene amplification without reverse transcription.Analytica chimica acta · 2022Article
- Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2.Sensors and actuators. B, Chemical · 2022Article
- Programmable High-Speed and Hyper-Efficiency DNA Signal Magnifier.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Single-tube isothermal label-free fluorescent sensor for pathogen detection based on genetic signatures.Frontiers in chemistry · 2022Article
- RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection.Scientific reports · 2018Article
- DNA nanotechnology for nucleic acid analysis: multifunctional molecular DNA machine for RNA detection.Chemical communications (Cambridge, England) · 2016Article
- Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification.Scientific reports · 2016Article
- A short antisense oligonucleotide ameliorates symptoms of severe mouse models of spinal muscular atrophy.Molecular therapy. Nucleic acids · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, we developed a simple isothermal nucleic acid amplification reaction, primer generation-rolling circle amplification (PG-RCA), to detect specific DNA sequences with great sensitivity and large dynamic range. In this paper, we combined PG-RCA with a three-way junction (3WJ) formation, and detected specific RNA molecules with high sensitivity and specificity in a one-step and isothermal reaction format. In the presence of target RNA, 3WJ probes (primer and template) are designed to form a 3WJ structure, from which multiple signal primers for the following PG-RCA can be generated by repeating primer extension, nicking and signal primer dissociation. Although this signal primer generation is a linear amplification process, the PG-RCA exponentially can amplify these signal primers and thus even a very small amount of RNA specimen can be detected. After optimizing the structures of 3WJ probes, the detection limit of this assay was 15.9 zmol (9.55 × 10(3) molecules) of synthetic RNA or 143 zmol (8.6 × 10(4) molecules) of in vitro transcribed human CD4 mRNA. Further, the applicability of this assay to detect CD4 mRNA in a human mRNA sample was demonstrated.
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