ArticleMolecular genetics and genomics : MGG2012
Molecular characterization and functional analysis by heterologous expression in E. coli under diverse abiotic stresses for OsLEA5, the atypical hydrophobic LEA protein from Oryza sativa L.
Article in Molecular genetics and genomics : MGG, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 84 citations in OpenAlex.
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- Recent Molecular Aspects and Integrated Omics Strategies for Understanding the Abiotic Stress Tolerance of Rice.Plants (Basel, Switzerland) · 2023Review
- Enhancement of Heat and Drought Stress Tolerance in Rice by Genetic Manipulation: A Systematic Review.Rice (New York, N.Y.) · 2022Review
- Combining GWAS, Genome-Wide Domestication and a Transcriptomic Analysis Reveals the Loci and Natural Alleles of Salt Tolerance in Rice (Frontiers in plant science · 2022Article
- Overexpression of rice OsLEA5 relieves the deterioration in seed quality caused by high-temperature stress.Plant biotechnology (Tokyo, Japan) · 2021Article
- Genome-wide identification and expression analysis of late embryogenesis abundant protein-encoding genes in rye (Secale cereale L.).PloS one · 2021Article
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- Characterization of natural genetic variation identifies multiple genes involved in salt tolerance in maize.Functional & integrative genomics · 2020Article
- Overexpression of OsLea14-A improves the tolerance of rice and increases Hg accumulation under diverse stresses.Environmental science and pollution research international · 2019Article
- Dissecting the Genomic Diversification of Late Embryogenesis Abundant (LEA) Protein Gene Families in Plants.Genome biology and evolution · 2019Article
- MiningInternational journal of molecular sciences · 2018Article
- Cotton Late Embryogenesis Abundant (G3 (Bethesda, Md.) · 2018Article
- Drought Induced Signaling in Rice: Delineating Canonical and Non-canonical Pathways.Frontiers in chemistry · 2018Review
- LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii.Cell stress & chaperones · 2017Article
- The CsHSP17.2 molecular chaperone is essential for thermotolerance in Camellia sinensis.Scientific reports · 2017Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In this study, we report the molecular characterization and functional analysis of OsLEA5 gene, which belongs to the atypical late embryogenesis abundant (LEA) group 5C from Oryza sativa L. The cDNA of OsLEA5 contains a 456 bp ORF encoding a polypeptide of 151 amino acids with a calculated molecular mass of 16.5 kDa and a theoretical pI of 5.07. The OsLEA5 polypeptide is rich in Leu (10%), Ser (8.6%), and Asp (8.6%), while Cys, Trp, and Gln residue contents are very low, which are 2, 1.3, and 1.3%, respectively. Bioinformatic analysis revealed that group 5C LEA protein subfamily contains a Pfam:LEA_2 domain architecture and is highly hydrophobic, intrinsically ordered with largely β-sheet and specific amino acid composition and distribution. Real-time PCR analysis showed that OsLEA5 was expressed in different tissue organs during different development stages of rice. The expression levels of OsLEA5 in the roots and panicles of full ripe stage were dramatically increased. The results of stress tolerance and cell viability assay demonstrated that recombinant E. coli cells producing OsLEA5 fusion protein exhibited improved resistance against diverse abiotic stresses: high salinity, osmotic, freezing, heat, and UV radiation. The OsLEA5 protein confers stabilization of the LDH under different abiotic stresses, such as heating, freeze-thawing, and drying in vitro. The combined results indicated that OsLEA5 protein was a hydrophobic atypical LEA and closely associated with resistance to multiple abiotic stresses. This research offered the valuable information for the development of crops with enhanced resistance to diverse stresses.
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