ArticleNature methods2011
Directed molecular evolution to design advanced red fluorescent proteins.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 76 citations in OpenAlex.
- Development of far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging.Cell reports methods · 2026Article
- Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging.bioRxiv : the preprint server for biology · 2025Article
- Dual-expression system for blue fluorescent protein optimization.Scientific reports · 2022Article
- Rapid directed molecular evolution of fluorescent proteins in mammalian cells.Protein science : a publication of the Protein Society · 2022Article
- Enhanced small green fluorescent proteins as a multisensing platform for biosensor development.Frontiers in bioengineering and biotechnology · 2022Article
- LSSmScarlet, dCyRFP2s, dCyOFP2s and CRISPRed2s, Genetically Encoded Red Fluorescent Proteins with a Large Stokes Shift.International journal of molecular sciences · 2021Article
- Advanced imaging and labelling methods to decipher brain cell organization and function.Nature reviews. Neuroscience · 2021Review
- FRCaMP, a Red Fluorescent Genetically Encoded Calcium Indicator Based on Calmodulin from Schizosaccharomyces Pombe Fungus.International journal of molecular sciences · 2020Article
- Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein.International journal of molecular sciences · 2020Article
- Expression and Characterization of a Bright Far-red Fluorescent Protein from the Pink-Pigmented Tissues of Porites lobata.Marine biotechnology (New York, N.Y.) · 2020Article
- Near-Infrared Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial Phytochrome.International journal of molecular sciences · 2019Article
- Slowly Reducible Genetically Encoded Green Fluorescent Indicator for In Vivo and Ex Vivo Visualization of Hydrogen Peroxide.International journal of molecular sciences · 2019Article
- Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins.International journal of molecular sciences · 2018Article
- NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics.Scientific reports · 2018Article
- A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.Nature chemical biology · 2018Article
- Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion.Biophysics reports · 2018Article
- New imaging probes to track cell fate: reporter genes in stem cell research.Cellular and molecular life sciences : CMLS · 2017Review
- Article
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
- Peculiarities of the Super-Folder GFP Folding in a Crowded Milieu.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins. We discuss possibilities for knowledge-based rational design based on the photochemistry of fluorescent proteins and the position of the chromophore in protein structure. We consider advances in library design by mutagenesis, protein expression systems and instrumentation for high-throughput screening that should yield improved fluorescent proteins for advanced imaging applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.