ArticleMolecular & cellular proteomics : MCP2012
Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 70 citations in OpenAlex.
- Cyclic stretch increases transglutaminase 2-mediated serotonylation of extracellular fibronectin in cultured optic nerve head astrocytes: a pilot study.Matrix biology plus · 2026Article
- Stretch strain shifts optic nerve head astrocyte metabolism to enrich α-ketoglutarate and the pentose phosphate pathway.Scientific reports · 2026Article
- Müller glia-mediated protection of retinal ganglion cells from ethambutol-induced neurotoxicity through brain-derived neurotrophic factor.Cell communication and signaling : CCS · 2025Article
- Mechanobiology in the eye.npj biological physics and mechanics · 2025Review
- Extracellular vesicles as emerging players in glaucoma: Mechanisms, biomarkers, and therapeutic targets.Vision research · 2025Review
- Optic Nerve Head Pulsatile Displacement in Open-Angle Glaucoma after Intraocular Pressure Reduction Measured by Optical Coherence Tomography: A Pilot Study.Bioengineering (Basel, Switzerland) · 2024Article
- Role of Epithelial-to-Mesenchymal Transition of Retinal Pigment Epithelial Cells in Glaucoma Cupping.Journal of clinical medicine · 2023Article
- Evidence of an Annexin A4 mediated plasma membrane repair response to biomechanical strain associated with glaucoma pathogenesis.Journal of cellular physiology · 2022Article
- Remodeling of the Lamina Cribrosa: Mechanisms and Potential Therapeutic Approaches for Glaucoma.International journal of molecular sciences · 2022Review
- Microstructural Deformations Within the Depth of the Lamina Cribrosa in Response to Acute In Vivo Intraocular Pressure Modulation.Investigative ophthalmology & visual science · 2022Article
- CCN2/CTGF-A Modulator of the Optic Nerve Head Astrocyte.Frontiers in cell and developmental biology · 2022Article
- Stretch stress propels glutamine dependency and glycolysis in optic nerve head astrocytes.Frontiers in neuroscience · 2022Article
- An inducible rodent glaucoma model that exhibits gradual sustained increase in intraocular pressure with distinct inner retina and optic nerve inflammation.Scientific reports · 2021Article
- Multiplex protein analysis for the study of glaucoma.Expert review of proteomics · 2021Review
- Decorin-An Antagonist of TGF-β in Astrocytes of the Optic Nerve.International journal of molecular sciences · 2021Article
- Mirna Expression in Glaucomatous and TGFβ2 Treated Lamina Cribrosa Cells.International journal of molecular sciences · 2021Article
- Fractalkine, sICAM-1 and Kynurenine Pathway in Restrictive Anorexia Nervosa-Exploratory Study.Nutrients · 2021Article
- Biomechanics of the optic nerve head and peripapillary sclera in a mouse model of glaucoma.Journal of the Royal Society, Interface · 2020Article
- Lamina Cribrosa Capillaries Straighten as Intraocular Pressure Increases.Investigative ophthalmology & visual science · 2020Article
- Isolation and characterization of human optic nerve head astrocytes and lamina cribrosa cells.Experimental eye research · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We investigate the role of glial cell activation in the human optic nerve caused by raised intraocular pressure, and their potential role in the development of glaucomatous optic neuropathy. To do this we present a proteomics study of the response of cultured, optic nerve head astrocytes to biomechanical strain, the magnitude and mode of strain based on previously published quantitative models. In this case, astrocytes were subjected to 3 and 12% stretches for either 2 h or 24 h. Proteomic methods included nano-liquid chromatography, tandem mass spectrometry, and iTRAQ labeling. Using controls for both stretch and time, a six-plex iTRAQ liquid chromatography- tandem MS (LC/MS/MS) experiment yielded 573 proteins discovered at a 95% confidence limit. The pathways included transforming growth factor β1, tumor necrosis factor, caspase 3, and tumor protein p53, which have all been implicated in the activation of astrocytes and are believed to play a role in the development of glaucomatous optic neuropathy. Confirmation of the iTRAQ analysis was performed by Western blotting of various proteins of interest including ANXA 4, GOLGA2, and αB-Crystallin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.