ArticlePLoS genetics2011
Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Epistasis and co-adaptation in bacterial genome evolution.Nature reviews. Genetics · 2026Review
- TXA11114: Discovery of anAntibiotics (Basel, Switzerland) · 2026Article
- An integrative multi-omics approach points to membrane composition as a key factor in E. coli persistence.PloS one · 2026Article
- Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle.Cell systems · 2025Article
- Lytic Transglycosylase Deficiency Increases Susceptibility to β-lactam Antibiotics But Reduces Susceptibility to Vancomycin in Escherichia coli.Microbiology and immunology · 2025Article
- The interplay betweenInfection and immunity · 2025Article
- Whole genome CRISPRi screening identifies druggable vulnerabilities in an isoniazid resistant strain of Mycobacterium tuberculosis.Nature communications · 2024Article
- Investigating the Activities ofBiology · 2024Article
- Cymoxanil disrupts RNA synthesis through inhibiting the activity of dihydrofolate reductase.Scientific reports · 2024Article
- A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli.Cell reports methods · 2024Article
- Article
- Interaction networks of Escherichia coli replication proteins under different bacterial growth conditions.Scientific data · 2023Article
- High-throughput functional genomics: A (myco)bacterial perspective.Molecular microbiology · 2023Review
- Article
- Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli.Nature communications · 2022Article
- Evaluation of a Conformationally Constrained Indole Carboxamide as a Potential Efflux Pump Inhibitor in Pseudomonas aeruginosaAntibiotics (Basel, Switzerland) · 2022Article
- Article
- Comparative genomic analyses reveal genetic characteristics and pathogenic factors ofFrontiers in microbiology · 2022Article
- Homeostasis of the Gram-negative cell envelope.Current opinion in microbiology · 2021Review
- Comprehensive genetic analysis of adhesin proteins and their role in virulence of Candida albicans.Genetics · 2021Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
23 authors.
Funding
Abstract
As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target.
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