ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Transient muscle paralysis degrades bone via rapid osteoclastogenesis.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Early Biological Orchestration in Fracture Healing: Decoding the Crosstalk between Inflammation, Coagulation, and Metabolism.Stem cell reviews and reports · 2026Review
- Exercise-responsive crosstalk within local muscle-bone units in osteosarcopenia: from mechanotransduction to secretome signalling.Frontiers in physiology · 2026Review
- Predicting cortical bone resorption in the mouse tibia under disuse conditions caused by transient muscle paralysis.Scientific reports · 2025Article
- The Role of Botulinum Neurotoxin A in the Conservative Treatment of Fractures: An Experimental Study on Rats.TheScientificWorldJournal · 2024Article
- A microCT-based platform to quantify drug targeting.European radiology experimental · 2023Article
- Basolateral Amygdala Mediates Central Mechanosensory Feedback of Musculoskeletal System.Frontiers in molecular neuroscience · 2022Article
- The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats.TheScientificWorldJournal · 2022Article
- Inflammation, Oxidative Stress, and Bone in Chronic Kidney Disease in the Osteoimmunology Era.Calcified tissue international · 2021Review
- Article
- The effects on the mandibular condyle of Botox injection into the masseter are not transient.American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics · 2019Article
- Neuromuscular dysfunction, independent of gait dysfunction, modulates trabecular bone homeostasis in mice.Journal of musculoskeletal & neuronal interactions · 2019Article
- Impact of muscle atrophy on bone metabolism and bone strength: implications for muscle-bone crosstalk with aging and disuse.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2018Review
- Origin of Reparative Stem Cells in Fracture Healing.Current osteoporosis reports · 2018Review
- Muscle paralysis induces bone marrow inflammation and predisposition to formation of giant osteoclasts.American journal of physiology. Cell physiology · 2017Article
- Pantoprazole, a proton pump inhibitor, does not prevent botulinum toxin induced disuse osteopenia in mice.Journal of musculoskeletal & neuronal interactions · 2017Article
- Bone and cartilage changes in rabbit mandibular condyles after 1 injection of botulinum toxin.American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics · 2015Article
- Immobilization induced osteopenia is strain specific in mice.Bone reports · 2015Article
- Muscle-bone interactions during fracture healing.Journal of musculoskeletal & neuronal interactions · 2015Review
- Biomechanical aspects of the muscle-bone interaction.Current osteoporosis reports · 2015Review
- Pulsed focused ultrasound treatment of muscle mitigates paralysis-induced bone loss in the adjacent bone: a study in a mouse model.Ultrasound in medicine & biology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
A unilateral injection of botulinum toxin A (BTxA) in the calf induces paralysis and profound loss of ipsalateral trabecular bone within days. However, the cellular mechanism underlying acute muscle paralysis-induced bone loss (MPIBL) is poorly understood. We hypothesized that MPIBL arises via rapid and extensive osteoclastogenesis. We performed a series of in vivo experiments to explore this thesis. First, we observed elevated levels of the proosteoclastogenic cytokine receptor activator for nuclear factor-κB ligand (RANKL) within the proximal tibia metaphysis at 7 d after muscle paralysis (+113%, P<0.02). Accordingly, osteoclast numbers were increased 122% compared with the contralateral limb at 5 d after paralysis (P=0.04) and MPIBL was completely blocked by treatment with human recombinant osteoprotegerin (hrOPG). Further, conditional deletion of nuclear factor of activated T-cells c1 (NFATc1), the master regulator of osteoclastogenesis, completely inhibited trabecular bone loss (-2.2±11.9%, P<0.01). All experiments included negative control assessments of contralateral limbs and/or within-animal pre- and postintervention imaging. In summary, transient muscle paralysis induced acute RANKL-mediated osteoclastogenesis resulting in profound local bone resorption. Elucidation of the pathways that initiate osteoclastogenesis after paralysis may identify novel targets to inhibit bone loss and prevent fractures.
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