ArticleAnnals of biomedical engineering2012
Single-detector simultaneous optical mapping of V(m) and [Ca(2+)](i) in cardiac monolayers.
Article in Annals of biomedical engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Tissue elasticity modulates cardiac pacemaker cell automaticity.American journal of physiology. Heart and circulatory physiology · 2025Article
- Methodology for Cross-Talk Elimination in Simultaneous Voltage and Calcium Optical Mapping Measurements With Semasbestic Wavelengths.Frontiers in physiology · 2022Article
- In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy.Methods in enzymology · 2021Article
- Parallel Acceleration on Removal of Optical Mapping Baseline Wandering.Computing in cardiology · 2019Article
- Open-Source Multiparametric Optocardiography.Scientific reports · 2019Article
- Lights, camera, path splitter: a new approach for truly simultaneous dual optical mapping of the heart with a single camera.BMC biomedical engineering · 2019Article
- Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.Nature protocols · 2018Article
- Fibroblasts Slow Conduction Velocity in a Reconstituted Tissue Model of Fibrotic Cardiomyopathy.ACS biomaterials science & engineering · 2017Article
- Effects of laser polarization on responses of the fluorescent CaNeurophotonics · 2017Article
- Cardiac tissue slices: preparation, handling, and successful optical mapping.American journal of physiology. Heart and circulatory physiology · 2015Article
- Cardiac electrophysiological imaging systems scalable for high-throughput drug testing.Pflugers Archiv : European journal of physiology · 2012Article
- Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques.Pflugers Archiv : European journal of physiology · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Simultaneous mapping of transmembrane voltage (V(m)) and intracellular Ca(2+) concentration (Ca(i)) has been used for studies of normal and abnormal impulse propagation in cardiac tissues. Existing dual mapping systems typically utilize one excitation and two emission bandwidths, requiring two photodetectors with precise pixel registration. In this study we describe a novel, single-detector mapping system that utilizes two excitation and one emission band for the simultaneous recording of action potentials and calcium transients in monolayers of neonatal rat cardiomyocytes. Cells stained with the Ca(2+)-sensitive dye X-Rhod-1 and the voltage-sensitive dye Di-4-ANEPPS were illuminated by a programmable, multicolor LED matrix. Blue and green LED pulses were flashed 180° out of phase at a rate of 488.3 Hz using a custom-built dual bandpass excitation filter that transmitted blue (482 ± 6 nm) and green (577 ± 31 nm) light. A long-pass emission filter (>605 nm) and a 504-channel photodiode array were used to record combined signals from cardiomyocytes. Green excitation yielded Ca(i) transients without significant crosstalk from V(m). Crosstalk present in V(m) signals obtained with blue excitation was removed by subtracting an appropriately scaled version of the Ca(i) transient. This method was applied to study delay between onsets of action potentials and Ca(i) transients in anisotropic cardiac monolayers.
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Registered trials
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