ArticleApplied microbiology and biotechnology2012
Computational identification of gene over-expression targets for metabolic engineering of taxadiene production.
Article in Applied microbiology and biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
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- FluxRETAP: a REaction TArget Prioritization genome-scale modeling technique for selecting genetic targets.Bioinformatics (Oxford, England) · 2025Article
- Enhanced anaerobic synthesis of rhamnolipids and emulsification of crude oil by genetically engineered Pseudomonas aeruginosa strains.Microbial cell factories · 2025Article
- Genome-scale metabolic model led engineering ofFrontiers in plant science · 2023Article
- Metabolic engineering of3 Biotech · 2021Article
- A Computational Framework to Identify Metabolic Engineering Strategies for the Co-Production of Metabolites.Frontiers in bioengineering and biotechnology · 2021Article
- Reconstruction and Analysis of a Genome-Scale Metabolic Model ofFrontiers in microbiology · 2018Article
- Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus.Microbial cell factories · 2017Article
- Reconstruction of a Genome-scale Metabolic Network of Komagataeibacter nataicola RZS01 for Cellulose Production.Scientific reports · 2017Article
- Insights into the metabolic mechanism of rapamycin overproduction in the shikimate-resistant Streptomyces hygroscopicus strain UV-II using comparative metabolomics.World journal of microbiology & biotechnology · 2017Article
- Enhancement of rapamycin production by metabolic engineering in Streptomyces hygroscopicus based on genome-scale metabolic model.Journal of industrial microbiology & biotechnology · 2017Article
- Reconstruction and analysis of the genome-scale metabolic model of schizochytrium limacinum SR21 for docosahexaenoic acid production.BMC genomics · 2015Article
- Identification of a critical determinant that enables efficient fatty acid synthesis in oleaginous fungi.Scientific reports · 2015Article
- Reconstruction and analysis of a genome-scale metabolic model of the oleaginous fungus Mortierella alpina.BMC systems biology · 2015Article
- Article
- Partial inhibition and bilevel optimization in flux balance analysis.BMC bioinformatics · 2013Article
- Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement.Microbial cell factories · 2013Article
- The early stages of taxol biosynthesis: an interim report on the synthesis and identification of early pathway metabolites.Natural product reports · 2012Review
- Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds.Applied and environmental microbiology · 2012Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Taxadiene is the first dedicated intermediate in the biosynthetic pathway of the anticancer compound Taxol. Recent studies have taken advantage of heterologous hosts to produce taxadiene and other isoprenoid compounds, and such ventures now offer research opportunities that take advantage of the engineering tools associated with the surrogate host. In this study, metabolic engineering was applied in the context of over-expression targets predicted to improve taxadiene production. Identified targets included genes both within and outside of the isoprenoid precursor pathway. These targets were then tested for experimental over-expression in a heterologous Escherichia coli host designed to support isoprenoid biosynthesis. Results confirmed the computationally predicted improvements and indicated a synergy between targets within the expected isoprenoid precursor pathway and those outside this pathway. The presented algorithm is broadly applicable to other host systems and/or product choices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.