ArticleMetabolic engineering2012
Remodeling the isoprenoid pathway in tobacco by expressing the cytoplasmic mevalonate pathway in chloroplasts.
Article in Metabolic engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 125 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Advances in Genome Sequencing and Natural Rubber Biosynthesis in Rubber-Producing Plants.Current issues in molecular biology · 2023Review
- Current Status and De Novo Synthesis of Anti-Tumor Alkaloids inMetabolites · 2023Review
- Changing biosynthesis of terpenoid percursors in rice through synthetic biology.Frontiers in plant science · 2023Article
- Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering.Synthetic and systems biotechnology · 2022Article
- Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice.Transgenic research · 2022Article
- Downregulation of Squalene Synthase Broadly Impacts Isoprenoid Biosynthesis in Guayule.Metabolites · 2022Article
- Mini-synplastomes for plastid genetic engineering.Plant biotechnology journal · 2022Article
- Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells.Plant communications · 2021Review
- Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis.Plant methods · 2021Article
- Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants.Journal of experimental botany · 2021Article
- Biosynthesis and Biological Activities of Newly Discovered Amaryllidaceae Alkaloids.Molecules (Basel, Switzerland) · 2020Review
- On the Evolution and Functional Diversity of Terpene Synthases in the Pinus Species: A Review.Journal of molecular evolution · 2020Review
- High-Level Patchoulol Biosynthesis inFrontiers in bioengineering and biotechnology · 2020Article
- Natural rubber biosynthesis in plants, the rubber transferase complex, and metabolic engineering progress and prospects.Plant biotechnology journal · 2019Review
- Functional Analysis of Polyprenyl Diphosphate Synthase Genes Involved in Plastoquinone and Ubiquinone Biosynthesis inFrontiers in plant science · 2019Article
- Review
- Physcomitrella patens, a versatile synthetic biology chassis.Plant cell reports · 2018Review
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
Abstract
Metabolic engineering to enhance production of isoprenoid metabolites for industrial and medical purposes is an important goal. The substrate for isoprenoid synthesis in plants is produced by the mevalonate pathway (MEV) in the cytosol and by the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway in plastids. A multi-gene approach was employed to insert the entire cytosolic MEV pathway into the tobacco chloroplast genome. Molecular analysis confirmed the site-specific insertion of seven transgenes and homoplasmy. Functionality was demonstrated by unimpeded growth on fosmidomycin, which specifically inhibits the MEP pathway. Transplastomic plants containing the MEV pathway genes accumulated higher levels of mevalonate, carotenoids, squalene, sterols, and triacyglycerols than control plants. This is the first time an entire eukaryotic pathway with six enzymes has been transplastomically expressed in plants. Thus, we have developed an important tool to redirect metabolic fluxes in the isoprenoid biosynthesis pathway and a viable multigene strategy for engineering metabolism in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.