ArticleJournal of bacteriology2012
Biochemical disclosure of the mycolate outer membrane of Corynebacterium glutamicum.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
- The mycomembrane proteins PorH and ProtX are inserted at polar growth zones and linked to the cell wall.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Engineering of Corynebacterium glutamicum for the synthesis of aromatic compounds.Applied microbiology and biotechnology · 2025Review
- Mapping the ultrastructural topology of the corynebacterial cell surface.PLoS biology · 2025Article
- Modulating mycobacterial envelope integrity for antibiotic synergy with benzothiazoles.Life science alliance · 2024Article
- Chemical Proteomics Strategies for Analyzing Protein Lipidation Reveal the BacterialJournal of the American Chemical Society · 2024Article
- Defining Membrane Protein Localization by Isopycnic Density Gradients.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Effects of benzothiazinone and ethambutol on the integrity of the corynebacterial cell envelope.Cell surface (Amsterdam, Netherlands) · 2023Article
- Article
- Identification and architecture of a putative secretion tube across mycobacterial outer envelope.Science advances · 2021Article
- Extracellular heme recycling and sharing across species by novel mycomembrane vesicles of a Gram-positive bacterium.The ISME journal · 2021Article
- Phylogenomic Reappraisal of Fatty Acid Biosynthesis, Mycolic Acid Biosynthesis and Clinical Relevance Among Members of the GenusFrontiers in microbiology · 2021Article
- Photoactivatable Glycolipid Probes for Identifying Mycolate-Protein Interactions in Live Mycobacteria.Journal of the American Chemical Society · 2020Article
- Improvement of Surface-Enhanced Raman Scattering Method for Single Bacterial Cell Analysis.Frontiers in bioengineering and biotechnology · 2020Article
- A FRET-Based Fluorogenic Trehalose Dimycolate Analogue for Probing Mycomembrane-Remodeling Enzymes of Mycobacteria.ACS omega · 2019Article
- Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin.Microbial cell factories · 2018Article
- Identification of novel lipid modifications and intermembrane dynamics inJournal of lipid research · 2018Article
- Production of the compatible solute α-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum.Microbial cell factories · 2018Article
- Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system.Microbial cell factories · 2017Article
- Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane.Scientific reports · 2017Article
- Tailoring Trehalose for Biomedical and Biotechnological Applications.Pure and applied chemistry. Chimie pure et appliquee · 2017Article
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterineae is a specific suborder of Gram-positive bacteria that includes Mycobacterium tuberculosis and Corynebacterium glutamicum. The cell wall of these bacteria is composed of a heteropolymer of peptidoglycan (PG) linked to arabinogalactan (AG), which in turn is covalently associated with an atypical outer membrane, here called mycomembrane (M). The latter structure has been visualized by cryo-electron microscopy of vitreous sections, but its biochemical composition is still poorly defined, thereby hampering the elucidation of its physiological function. In this report, we show for the first time that the mycomembrane-linked heteropolymer of PG and AG (M-AG-PG) of C. glutamicum can be physically separated from the inner membrane on a flotation density gradient. Analysis of purified M-AG-PG showed that the lipids that composed the mycomembrane consisted almost exclusively of mycolic acid derivatives, with only a tiny amount, if any, of phospholipids and lipomannans, which were found with the characteristic lipoarabinomannans in the plasma membrane. Proteins associated with or inserted in the mycomembrane were extracted from M-AG-PG with lauryl-dimethylamine-oxide (LDAO), loaded on an SDS-PAGE gel, and analyzed by tandem mass spectrometry or by Western blotting. Sixty-eight different proteins were identified, 19 of which were also found in mycomembrane fragments released by the terminal-arabinosyl-transferase-defective ΔAftB strain. Almost all of them are predicted to contain a signal sequence and to adopt the characteristic β-barrel structure of Gram-negative outer membrane proteins. These presumed mycomembrane proteins include the already-known pore-forming proteins (PorA and PorB), 5 mycoloyltransferases (cMytA, cMytB, cMytC, cMytD, and cMytF), several lipoproteins, and unknown proteins typified by a putative C-terminal hydrophobic anchor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.