ArticleBMC research notes2011
Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Small Extracellular Vesicles Orchestrate Cisplatin-Induced Ototoxicity: Potential Biomarker and Targets Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Role of Platelets in the Stimulation of Neuronal Synaptic Plasticity, Electric Activity, and Oxidative Phosphorylation: Possibilities for New Therapy of Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2021Article
- An image-based flow cytometric approach to the assessment of the nucleus-to-cytoplasm ratio.PloS one · 2021Article
- Neuronal extracellular microRNAs miR-124 and miR-9 mediate cell-cell communication between neurons and microglia.Journal of neuroscience research · 2019Article
- Epigenetic and transgenerational mechanisms in infection-mediated neurodevelopmental disorders.Translational psychiatry · 2017Review
- MiRNA-124 is a link between measles virus persistent infection and cell division of human neuroblastoma cells.PloS one · 2017Article
- Article
- Inhibition of microRNA-34a prevents IL-1β-induced extracellular matrix degradation in nucleus pulposus by increasing GDF5 expression.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Temporal Heterogeneity Metrics in Apoptosis Induced by Anticancer Drugs.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
- MicroRNA-93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells.Cell proliferation · 2015Article
- Downregulation of microRNA-124 predicts poor prognosis in glioma patients.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2015Article
- Dysfunctional HIV-specific CD8+ T cell proliferation is associated with increased caspase-8 activity and mediated by necroptosis.Immunity · 2014Article
- Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Brain microRNAs and insights into biological functions and therapeutic potential of brain enriched miRNA-128.Molecular cancer · 2014Review
- In vivo monitoring of microRNA biogenesis using reporter gene imaging.Theranostics · 2013Review
- Therapeutic evaluation of microRNAs by molecular imaging.Theranostics · 2013Review
- Downregulation of microRNA-124 is an independent prognostic factor in patients with colorectal cancer.International journal of colorectal disease · 2013Article
- Review
- Article
- Imaging flow cytometry: coping with heterogeneity in biological systems.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are regulatory molecules that play an important role in many physiological processes, including cell growth, differentiation, and apoptosis. In addition to modulating normal cellular functions, it has also been reported that miRNAs are involved in the development of many pathologies, including cardiovascular diseases, cancer, inflammation, and neurodegeneration. Methods for the sensitive detection and measurement of specific miRNAs and their cellular targets are essential for both basic research endeavours, as well as diagnostic efforts aimed at understanding the role of miRNAs in disease processes.
findingsIn this study, we describe a novel, imaging cytometry-based protocol that allows for simultaneous visualisation and quantification of miRNAs and their putative targets. We validated this methodology in a neuronal cell line by examining the relationship of the miRNA miR-124 and its known target, cyclin dependent kinase 6 (CDK6). We found that ectopic overexpression of miR-124 resulted in the downregulation of CDK6, decreased cellular proliferation, and induced cellular morphological changes.
conclusionsThis method is suitable for analysing the expression and cellular localisation of miRNAs and target proteins in small cell subsets within a heterogeneous cell suspension. We believe that our cytometry-based methodology will be easily adaptable to miRNA studies in many areas of biomedical research including neuroscience, stem cell biology, immunology, and oncology.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.