ArticleMolecular & cellular proteomics : MCP2012
Glutathionylation in the photosynthetic model organism Chlamydomonas reinhardtii: a proteomic survey.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 158 citations in OpenAlex.
- Regulation of plant glycolysis and the tricarboxylic acid cycle by posttranslational modifications.The Plant journal : for cell and molecular biology · 2025Review
- Redox regulation of glutamate-1-semialdehyde aminotransferase modulates the synthesis of 5-aminolevulinic acid in Arabidopsis.Frontiers in plant science · 2025Article
- Nitric oxide regulates photosynthesis and the capacity of the antioxidant system under water deficit and rehydration inFrontiers in plant science · 2025Article
- Post-Translational Modifications to Cysteine Residues in Plant Proteins and Their Impact on the Regulation of Metabolism and Signal Transduction.International journal of molecular sciences · 2024Review
- The activation ofPlant direct · 2024Article
- Plant Life with and without Oxygen: A Metabolomics Approach.International journal of molecular sciences · 2023Review
- Photorespiration: regulation and new insights on the potential role of persulfidation.Journal of experimental botany · 2023Review
- Redox regulation, thioredoxins, and glutaredoxins in retrograde signalling and gene transcription.Journal of experimental botany · 2023Review
- The pyrenoid: the eukaryotic CO2-concentrating organelle.The Plant cell · 2023Article
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- Novel cost-effective design for bio-volatilization studies in photosynthetic microalgae exposed to arsenic with emphasis on growth and glutathione modulation.Frontiers in microbiology · 2023Article
- Bioactive compounds by microalgae and potentials for the management of some human disease conditions.AIMS microbiology · 2023Review
- Animal models of male subfertility targeted on LanCL1-regulated spermatogenic redox homeostasis.Lab animal · 2022Article
- Scavenging of nitric oxide up-regulates photosynthesis under drought in Festuca arundinacea and F. glaucescens but reduces their drought tolerance.Scientific reports · 2022Article
- Heavy metal-induced stress in eukaryotic algae-mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response.Environmental science and pollution research international · 2022Review
- Redox regulation of enzymes involved in sulfate assimilation and in the synthesis of sulfur-containing amino acids and glutathione in plants.Frontiers in plant science · 2022Review
- Reduction in chloroplastic ribulose-5-phosphate-3-epimerase decreases photosynthetic capacity in Arabidopsis.Frontiers in plant science · 2022Article
- Chlamydomonas as a model for reactive oxygen species signaling and thiol redox regulation in the green lineage.Plant physiology · 2021Review
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- Protein Carbonylation: Emerging Roles in Plant Redox Biology and Future Prospects.Plants (Basel, Switzerland) · 2021Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein glutathionylation is a redox post-translational modification occurring under oxidative stress conditions and playing a major role in cell regulation and signaling. This modification has been mainly studied in nonphotosynthetic organisms, whereas much less is known in photosynthetic organisms despite their important exposure to oxidative stress caused by changes in environmental conditions. We report a large scale proteomic analysis using biotinylated glutathione and streptavidin affinity chromatography that allowed identification of 225 glutathionylated proteins in the eukaryotic unicellular green alga Chlamydomonas reinhardtii. Moreover, 56 sites of glutathionylation were also identified after peptide affinity purification and tandem mass spectrometry. The targets identified belong to a wide range of biological processes and pathways, among which the Calvin-Benson cycle appears to be a major target. The glutathionylation of four enzymes of this cycle, phosphoribulokinase, glyceraldehyde-3-phosphate dehydrogenase, ribose-5-phosphate isomerase, and phosphoglycerate kinase was confirmed by Western blot and activity measurements. The results suggest that glutathionylation could constitute a major mechanism of regulation of the Calvin-Benson cycle under oxidative stress conditions.
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